摘要
目的分析2013年珠海市手足口病（Hand-foot-mouth disease,HFMD）患者中,肠道病毒71型（EV71）和柯萨奇病毒A组16型（Cox A16）的分子流行病学特征。方法采集珠海市2013年HFMD临床诊断病例粪便标本215份,选取其中Real time RT-PCR检测EV71病毒核酸阳性的18份和Cox A16病毒核酸阳性的8份标本,进行VP4区基因扩增,并进行核苷酸序列和遗传进化关系分析。结果 16份EV71扩增测序成功,与C4a基因亚型代表株具有最高的核苷酸序列同源性（95.2%-99.5%）和氨基酸同源性（91.3%-100%）;5份Cox A16扩增测序成功,有2份与B2a亚型代表株具有最高的核苷酸序列同源性（98.6%-99.0%）和氨基酸同源性（97.1%-98.5%）,3份与B2b亚型代表株具有最高的核苷酸序列同源性（96.6%-97.1%）和氨基酸同源性（100%）。结论 2013年珠海市流行的EV71属于C4a基因亚型,Cox A16为B2a和B2b两种基因亚型。
Objective To analyze molecular epidemiological characteristics of enterovirus 71 （EV71） and coxsackie virus A16 （CoxA16） isolated from specimens of patients with hand-foot-mouth disease （HFMD）in Zhuhai in 2013. Methods The 215 HFMD specimens were collected from Zhuhai in 2013 and EV71 and CoxA16 were detected by real time RT-PCR. The whole VP4 genes of 18 EV71 positive specimens and 8 CoxA16 positive ones were amplified by RT-PCR and sequenced, and compared with other isolates available from Genbank by homogeneity and phylogenetic tree analyses. Results Sixteen of the 18 EV71 positive specimens were amplified and sequenced successfully, and clustered within the C4a. The nucleotide and amino acid homogeneity of them with the representative isolate of C4a were 95.2%-99.5% and 91.3%-100%, respectively. Five of eight CoxA16 positive specimens were amplified and sequenced successfully, and 2 ones formed a distinct cluster in subgenotype B2a, with the nueleotide homogeneity and mino acid homogeneity oi98.6%-99.0% and 97.1%-98.5%, the other 3 ones formed a distinct duster in subgenotype B2b, with nuclootide homogeneity and mino acid homogeneity of 96.6%-97.1% and 100% accordingly. Conclusion The EV71 and CoxA16 prevalent in Zhuhai in 2013 belonged to C4a subgenotype and B2a and B2b subgenotypes.
出处
《中国热带医学》
CAS
2014年第12期1429-1434,共6页
China Tropical Medicine
基金
珠海市卫生局医学科技项目(No.2014064)
