胰高血糖素样肽-1在中医振腹疗法治疗2型糖尿病中的机制探讨

Study on Mechanism of TCM Abdominal Vibration Therapy Curing Type2 Diabetes Mellitus via GLP-1 Pathway

目的:探究中医振腹疗法在治疗2型糖尿病(Type 2 Diabetes Mellitus,T2DM)中对胰高血糖素样肽-1(Glucagon-Like Peptide-1,GLP-1)及大鼠糖代谢的影响及机制。方法:采用长期高糖高脂饲料饲养加小剂量链脲佐菌素(STZ,30 mg·kg-1,腹腔注射)诱导2型糖尿病大鼠模型,动物随机分为空白对照组、模型组、西药组、振腹组、西药加振腹组。结果:与模型组比较,振腹组体重变化量明显减小(P<0.05),西药加振腹组体重变化量与西药组比较明显减小(P<0.05)。治疗后振腹组大鼠血糖值较模型组显著降低(P<0.05)。振腹组和西药加振腹组GLP-1含量较模型组显著升高(P<0.05),西药加振腹组GLP-1含量较西药组升高(P<0.05)。各组病理结果显示:西药加振腹组的大鼠胰腺破坏的程度最低,其次是振腹组、西药组、模型组。结论:振腹疗法可以降低血糖,改善糖尿病症状,减轻或减缓胰岛细胞破坏,其降糖作用可能与GLP-1途径有关。

Objective： The study was to explore the effect of TCM abdominal vibration treatment on glucagon-like peptide-1（ GLP-1）and rats glucose metabolism. Methods： To induced animal models of type 2 diabetes with Long-term high glucose and lipid diet and a small dose of streptozotocin（ STZ,30mg·kg^-1,ip）. Rats were randomly divided into control group,model group,western medicine group,abdominal vibration group,western medicine plus abdominal vibration group. Results： After treatment,the weight of abdominal vibration group was significantly reduced compared to T2 DM model group. Weight reduction was more significant in western medicine plus abdominal vibration group than in western medicine group（ P〈0. 05）. GLP-1 increased significantly in abdominal vibration group,western medicine group,western plus abdominal vibration group（ P〈0. 05）. The pathological slice of Islet beta cell showed that western medicine plus abdominal vibration group was least damaged,followed by abdominal vibration group,western medicine group and model group. Conclusion： Abdominal vibration therapy can reduce serum glucose,relieve diabetes symptoms and cell damage via GLP-1 pathway.