摘要
目的建立HPLC法同时测定药用辅料苯甲醇中苯甲酸和苯甲醛的含量。方法采用Acclaim 120 C18柱（4.6 mm×250 mm,5μm）,流动相为乙腈-0.02%甲酸（用氨水调至p H=4.5）（30：70）,流速为1.0 m L·min^-1,检测波长为230 nm,柱温为35℃,进样量为20μL。结果苯甲酸在0.500 6-15.02μg·mL^-1内与峰面积线性关系良好,平均回收率均〉100%,RSD均〈1.3%（n=3）;苯甲醛在0.505 2-15.16μg·mL^-1内与峰面积的线性关系良好,平均回收率均〉108%,RSD均〈0.15%（n=3）。结论本方法准确度高、重现性好,可用于苯甲醇中苯甲酸和苯甲醛的含量测定。
Objective To establish an HPLC method for the determination of benzoic acid and benzaldehyde in pharmaceutical excipients benzyl alcohol. Methods The column was Acclaim 120 C18（4.6 mm×250 mm, 5 μm）, the mobile phase was composed of acetotrile and 0.02% formic acid（adjusted p H to 4.5 with aqua ammonia）（30 ：70）, the flow rate was 1.0 m L·min- 1, the detection wavelength was 230 nm, the temperature was 35 ℃ and the injection volume was 20 μL. Results There was a good linearity of benzoic acid and benzaldehyde at 0.500 6- 15.02 μg·mL^-1 and 0.505 2- 15.16 μg·mL^-1. The average recoveries were more than 100%（RSD 〈1.3%, n = 3） and 108%（RSD 〈0.15%, n = 3）. Conclusion This method is reproducible and accurate to determine the content of benzoic acid and benzaldehyde in benzyl alcohol.
出处
《中南药学》
CAS
2014年第12期1239-1241,共3页
Central South Pharmacy
