汉防己甲素对急性切口痛大鼠的镇痛作用研究

Research on analgesic effect of tetrandrine in rats with acute incision pain

目的探讨汉防己甲素对急性切口痛大鼠模型的镇痛作用。方法选择急性切口痛模型作为研究对象,将SD雄性大鼠30只随机分为急性疼痛组和汉防己甲素组,在术前1 d至术后6 d分别进行疼痛累积评分、热和机械痛阈测定。结果从切开后2 h^6 d急性疼痛模型组的热缩足反射潜伏期均显著低于基础值,但汉防己甲素组只有术后2 h^3 d热缩足反射潜伏期显著低于基础值(P<0.05),而且汉防己甲素组的热缩足反射潜伏期从切开后2 h^6 d均显著高于急性疼痛模型组(P<0.05)。急性疼痛模型组和汉防己甲素组的50%缩足阈值在术后2 h^6 d均显著低于基础值(P<0.05),两组间基础阈值差异无统计学意义(P>0.05),但从切开后2 h^5 d,汉防己甲素组的50%缩足阈值显著高于急性疼痛模型组(P<0.05),术后6 d差异无统计学意义(P>0.05)。急性疼痛模型组及汉防己甲素组的疼痛累积评分在切开后均较基础值显著增加(P<0.05),两组间基础值差异无统计学意义(P>0.05),但术后汉防己甲素组的评分低于对照组,第5、6天差异无统计学意义(P>0.05)。结论汉防己甲素可缓解急性疼痛模型的静息痛、热及机械刺激的诱发痛,有希望成为新的术后镇痛方法。

Objective To probe into the analgesic effect of tetrandrine in rats with acute incision pain. Methods The model of acute incision pain was selected as research object.Thirty SD male rats were randomly divided into acute pain group and Tetrandrine group.One day before operation and six days after surgery,accumulative scores of pain and pain threshold of heat and mechanical withdrawal threshold were determined. Results In the acute pain group,thermal withdrawal latency from 2 hours after incision to the sixth day was greatly lower in comparison with base values.However,in the tetrandrine group,thermal withdrawal latency from 2 hours to the third day after surgery was lower compared with base values（P〈0.05）.From 2 hours after incision to the sixth day,thermal withdrawal latency in the tetrandrine group was all the time remarkably higher than that in the model group（P〈0.05）.In the acute pain model group and tetrandrine group,50% paw withdrawal threshold from 2 hours to the sixth day after surgery were both lower in comparison with base values（P〈0.05）.There was no significant difference in basic threshold in the two groups（P〈0.05）.From 2 hours after incision to the fifth day,50% paw withdrawal threshold in the tetrandrine group was greatly higher than that in the acute pain model group（P〈0.05）.There was no significant difference in the sixth day after surgery in both groups（P〈0.05）.Accumulative pain scores after skin incision in the two groups obviously increased in comparison with base values（P〈0.05）,but base values between the two group didn′t show significant difference（P〈0.05）.The score after surgery in the tetrandrine group was lower than that in the control group,without significant difference in fifth or sixth day（P 0.05）. Conclusion Tetrandrine can alleviate rest pain and evoked pain by heat and mechanical stimulation in the acute pain model,which has a chance to become a new postoperative analgesic method.