溶剂体系下Lipozyme RM IM催化卵磷脂乙醇解及其他醇对乙醇解的影响

Lipozyme RM IM-catalyzed ethanolysis of phosphatidylcholine in n-hexane and effects of alcohols on ethanolysis

以正己烷为溶剂,大豆卵磷脂(phosphatidylcholine,PC)为原料,利用Lipozyme RM IM催化乙醇解反应制备溶血卵磷脂(LPC),通过单因素实验确定了适宜的反应条件:温度40℃,加酶量15%(以PC质量计),溶剂比1∶2(PC:正己烷,g∶mL),底物比1∶3(PC∶醇中羟基,mol∶mol),加水量1∶80(PC:水,g∶μL);在此条件下,反应16 h达到平衡,LPC转化率达93%。在此基础上,考察其他醇对卵磷脂乙醇解的影响,发现正丁醇和叔丁醇对PC乙醇解无明显地促进或抑制作用,1,2-丙二醇和丙三醇对PC乙醇解表现出抑制作用。

Lysophosphatidylcholine （LPC） was prepared through alcoholysis of soybean phosphatidylcholine （PC） catalyzed by Lipozyme RM IM in n-hexane. Based on single factor experiments, the optimal ethanolysis condi- tions were obtained as followed ： reaction temperature 40~C , lipase dosage 15% （ in mass of PC） , solvent ratio 1 ： 2 （PC： n-hexane, m/v）, substrate ratio 1：3 （PC： hydroxyl of alcohol, mol/mol） , the amount of water 1：80 （PC： H20, g/uL）. Under these conditions, the conversion of LPC was 93% after 16 h. Based on these optimal condi- tions, the effect of other alcohols on the ethanolysis of PC was studied, n-butanol and tert-butanol showed no signifi- cant changes on ethanolysis, while 1,2-propylene glycol and glycerol showed significant inhibiting on ethanolysis.