摘要
以正己烷为溶剂,大豆卵磷脂(phosphatidylcholine,PC)为原料,利用Lipozyme RM IM催化乙醇解反应制备溶血卵磷脂(LPC),通过单因素实验确定了适宜的反应条件:温度40℃,加酶量15%(以PC质量计),溶剂比1∶2(PC:正己烷,g∶mL),底物比1∶3(PC∶醇中羟基,mol∶mol),加水量1∶80(PC:水,g∶μL);在此条件下,反应16 h达到平衡,LPC转化率达93%。在此基础上,考察其他醇对卵磷脂乙醇解的影响,发现正丁醇和叔丁醇对PC乙醇解无明显地促进或抑制作用,1,2-丙二醇和丙三醇对PC乙醇解表现出抑制作用。
Lysophosphatidylcholine (LPC) was prepared through alcoholysis of soybean phosphatidylcholine (PC) catalyzed by Lipozyme RM IM in n-hexane. Based on single factor experiments, the optimal ethanolysis condi- tions were obtained as followed : reaction temperature 40~C , lipase dosage 15% ( in mass of PC) , solvent ratio 1 : 2 (PC: n-hexane, m/v), substrate ratio 1:3 (PC: hydroxyl of alcohol, mol/mol) , the amount of water 1:80 (PC: H20, g/uL). Under these conditions, the conversion of LPC was 93% after 16 h. Based on these optimal condi- tions, the effect of other alcohols on the ethanolysis of PC was studied, n-butanol and tert-butanol showed no signifi- cant changes on ethanolysis, while 1,2-propylene glycol and glycerol showed significant inhibiting on ethanolysis.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2014年第6期37-43,共7页
Food and Fermentation Industries
基金
河南省高等学校青年骨干教师资助计划项目(2011GGJS-079)
河南工业大学研究生教育创新计划项目(2012YJCX10)