AB-8大孔树脂动态吸附山葡萄渣白藜芦醇

Optimization of dynamic adsorption of AB-8 macroporous resin in grape pomace resveratrol by response surface methodology

研究在单因素的基础上,以白藜芦醇浓度、流速和pH值为自变量,以白藜芦醇含量为响应值进行了响应面分析,建立了相应数学模型并对吸附条件进行了优化,然后对AB-8大孔树脂的洗脱条件进行了研究。结果表明:白藜芦醇浓度、流速和pH均对吸附率影响极显著,得到AB-8大孔树脂吸附白藜芦醇的最佳工艺参数为白藜芦醇浓度7.85 mg/mL、流速1.43BV/h、pH 4.11,在此条件下,吸附率为95.03%。当采用体积分数75%乙醇以0.9BV/h的流速进行洗脱时,获得的白藜芦醇的纯度为46.92%。

The dynamic adsorption conditions of AB-8 macroporous resin, such as concentration of resveratrol, work speed of the infusion and pH, were investigated using response surface methodology on basis of single factor ex- periment. The model of a second order quadratic equation for the adsorption rate of AB-8 maeroporous resin was estab- lished to optimize the adsorption process. Then, desorption conditions of AB-8 macroporousresinAB-8 were also dis- cussed. The results indicated that concentration of resveratrol, work speed of the infusion and pH had a significant effect on the adsorption rate. The optimal parameters were 7.85mg/mL resveratrol concentration, work speed of the infusion of 1.43 BV/hand, pH of 4. 11 and the adsorption rate was 95.03%. The purity of resveratrol was 46.92% when eluting 0.9 BV with 75% ethanol for 1 hour.