邻苯二甲醛法(OPA)与高效液相色谱法(HPLC)测定降胆固醇的双歧杆菌的对比

The comparison of o-phthalalehpyde(OPA) method and high performance liquid chromatography(HPLC) in the determination of cholesterol-lowering bifidobacteria

为了测定实验室保藏的5株双歧杆菌体外降胆固醇能力,分别采用邻苯二甲醛法与高效液相色谱法测定培养基中胆固醇,对两种方法的线性、精密度和加标回收率进行了对比。5株双歧杆菌菌株的降胆固醇率在12.34%-28.57%,两种方法分别在0-0.20 mg/mL和0-0.70 mg/mL范围内呈现良好的线性关系,精密度相对标准偏差分别为0.32%（n=5）和0.96%（n=5）,加标回收率在99.33%-100.18%。整体比较,两种方法均可用于测定双歧杆菌的降胆固醇能力,不过邻苯二甲醛法更适合降胆固醇双歧杆菌的初筛检测。

In order to measure the degradation rate of cholesterol of five strains in vitro,o-phthalalehpyde（OPA） method and High performance liquid chromatography （HPLC） method were used to determine the cholesterol of the me- dium respectively. Linearity,precision and recovery between the two methods were compared. The results were as follows ： the degradation rate of cholesterol of five strains are among 12.34% -28.57% ,two methods both had good linearity in the rang of 0 -0.20 mg/mL and 0 - 0.70 mg/mL respectively,precision of relative standard deviation was 0.32% （n = 5 ） and 0.96% （ n = 5 ） ,the spike recoveries were both between 99.33% and 100.18%. Overall comparison indicated that both the OPA method and HPLC method can measure the degradation rate of cholesterol of bifidobacteria.