鸡爪皮胶原多肽对蘑菇酪氨酸酶及B16黑色素瘤细胞的作用

Effect of chicken feet skin collagen peptide on mushroom tyrosinase and B16 melanoma cell

研究分子质量低于3 kDa鸡爪皮胶原多肽对蘑菇蘑菇酪氨酸酶的抑制及对小鼠B16黑色素瘤细胞黑色素合成的影响。以L-多巴为底物,研究胶原肽对蘑菇酪氨酸酶抑制作用及抑制动力学。然后采用B16黑色素瘤细胞验证提取鸡爪皮胶原多肽的安全性及对黑色素合成影响的效果。结果表明,分子质量低于3 kDa的鸡爪皮胶原多肽抑制蘑菇酪氨酸酶的IC50值为0.570 mg/mL,抑制属于非竞争性抑制,米氏常数Km和抑制常数Ki分别为24.68μmol/mL和10.76 mg/mL。鸡爪皮胶原多肽在0~200μg/mL的质量浓度范围内对细胞的增殖活性没有影响,而且能够有效地抑制细胞黑色素的生成。

The effects of chicken leg skin collagen peptide with molecular weight of less than 3 kDa on mushroom tyrosinase and melanin biosynthesis in B16 melanoma cells were studied. The Collagen Peptide inhibitory activity on mushroom tyrosinase and its tyrosinase inhibition kinetics were investigated using L-DOPA as the substrate. Then the safety of chicken collagen peptide extract as well as its effect on the synthesis of melanin was verified using B16 mela- noma cells. The results showed that the chicken collagen polypeptide whose molecular weight less than 3 kDa had ty- rosinase inhibition activity with an IC50 value of 0. 570 mg/mL. In addition, the inhibition type belonged to noncom- petitive inhibition, and the Michaelis constant Km and inhibition constant Ki were 24.68 μmol/ml and 10.76 mg/ mL, respectively. Chicken collagen polypeptide in the concentration range of 0 -200 μg/mL had no effect on the pro- liferation of melanoma cells. Moreover, it could effectively suppress the generation of melanin cells.