低温蛋白酶MP发酵液离子交换树脂的脱色

The decolorization technique of cold-adapted protease fermented broth by ion exchange resin

低温蛋白酶MP发酵液中的色素沉积影响了其在产品中的应用,该文研究了利用离子交换树脂对其进行脱色。实验结果表明,阴离子树脂D352既能有效地脱色,又能保持较高的蛋白酶酶活;D352树脂脱色的最适pH值为6-7,最适温度为30℃,最有效的流速为6 BV/mL;D352树脂在吸附色素的同时也能吸附发酵液中大量杂蛋白分子,蛋白脱除率为45.2%;D352树脂在连续使用6次之后仍具有较强的脱色能力。

Many coloring substances existing in the fermented broth affected the application of cold-adapted pro- tease MP in the product appearance. In this paper the decolorization of protease fermented broth by ion exchange resin was studied. The results showed that anion resin D352 could not only effectively decolor but also maintain relative high enzyme activity. The optimum pH was 6 - 7, the optimum temperature was 30℃, and the optimum flow rate was 6 BV/mL. Anion resin D352 could absorb coloring substances as well as a large number of other proteins and the pro- tein removal rate was 45.2%. After 6-circle uses, anion resin D352 still had a good decoloring effects.