超声波协同复合酶提取兰州百合多糖

Study on the polysaccharide from Lilium davidii var. unicolor Salisb with ultrasonic-assisted compound enzyme hydrolysis method

为获得一种高效的兰州百合多糖的提取方法,采用超声波协同复合酶法,以兰州百合粉为原料,对复合酶的配比、酶解过程及超声提取过程进行了优化。结果表明,兰州百合多糖提取的最佳工艺条件是:凝乳酶加量1.5%,纤维素酶加量2.0%;超声温度50℃,超声时间25 min,料液比1∶25,超声功率225 W。此条件下兰州百合多糖提取率为39.860%,与超声波法、复合酶法提取兰州百合多糖相,比多糖提取率明显提高。

In order to obtain an extraction method for higher yield of Lilium davidii var. unicolor Salisb polysac- charide, the effect of uhrasonic-assisted compound enzyme hydrolysis method on the extraction yield of Lilium avidii var. unicolor Salisb was explored in this paper. The dosage of compound enzyme, the enzyme hydrolysis process and the ultrasonic extraction process were optimized. The optimum conditions were found as follows： chymosin 1.5% , cellulose 2.0% , ultrasonic temperature 50 ℃ , ultrasonic time 25 min, ultrasonic power 225 W, material-water ratio 1 ： 25. The yield of polysaccharide reached 39.86% , which was higher than that of the ultrasonic extraction method.