乳味饮料中5种农药的色谱法测定

Determination of five pesticides by HPLC in flavored milk drinks

低温冷冻萃取结合高效液相色谱法,测定乳味饮料中的苯磺隆、氟嘧磺隆、氟铃脲、氟苯脲、杀铃脲。反相C18柱分离,以V（甲醇）∶V（水）=73∶27为流动相,紫外可变波长检测。乙腈为萃取剂,-18℃冷冻萃取,考察了盐效应、冷冻时间、萃取剂与样品比例等条件对萃取结果的影响。优化条件下,5种农药的线性关系良好,相关系数大于0.999 4,相对标准偏差小于2.33%,加标回收率在92.3%~98.7%。

Subzero-temperature frozen extraction technique which using acetonitrile as a solvent was applied to the extraction of pesticide such as Tribenuron, Primisulfuron, Hexaflumuron, Teflubenzuron, Triflumuron from flavor- ed milk drinks. HPLC was used for the determination. The pesticide were separated by a reversed-phase C18 column and detected by Variable wavelength UV. The mobile phase was the mixture of methanol and water and the volume ratio was 73：27. Acetonitrile was used in extracting under 18 degrees below zero. Salt effect , frozen time and the volume ra- tio of extraction agent on the effect of extracting efficiency were studied. Under the optimal conditions, the linear regres- sion equations of five pesticide was good and the correlation coefficient was more than 0. 9994. The relative standard de- viations were less than 2. 333%. The recovery rates of the target analyte were between 92.3% and 98.7%.