荷移光谱法测定鸡肉中左氧氟沙星残留

Determination of levofloxacin residues in chicken by charge transfer spectrophotometry

采用荷移光谱法测定了鸡肉中左氧氟沙星残留量。左氧氟沙星与茜素在水与乙醇的混合溶液中发生反应,生成的荷移络合物的最大吸收波长为532 nm;表观摩尔吸光系数是6.47×103L/mol·cm;药物浓度在1-50 mg/L内符合比耳定律;鸡肉中左氧氟沙星的加标回收率为92.0%-96.8%,相对标准偏差为5.2%,方法检出限为1.0 mg/kg（按S/N=3计）。用红外光谱法探讨了反应机理。

A novel method for determination of Levofloxacin residues in chicken meat was developed based on charge transfer spectrophotometry, Levofloxacin and alizarin were reacted in mixed solution of water and ethanol. The complex compound had molar absorption of -6, 47 ×103L/（mol , era） at 532 nm. The medical concentration o- beyed the Beer＇s law at 1 -50 mg/L. The recovery was in the range of 92.0% -96. 8%, the RSDs of this method were less than 5.6% , the detection limits was 1, 0 mg/kg （S/N = 3）. The mechanism of the reaction was investiga- ted by infrared spectroscopy,