不同肽段抗体对IL-17抑制率的比较

Comparison of different peptide antibody inhibition rate of IL-17

目的:设计IL-17疫苗,将其免疫新西兰兔,生产并纯化出针对IL-17的抗体,通过检测抗体的结合率以及对IL-17功能的抑制率,筛选出一种对IL-17功能有高抑制率的疫苗。方法:筛选设计4条IL-17肽段(0001、0002、0003、0004),用设计的4个IL-17的肽段偶联后获得的疫苗免疫新西兰兔,产生针对不同肽段的抗体,用免疫亲和层析的方法,分离纯化抗IL-17抗体;用ELISA检测抗体与IL-17的结合,并对抗体进行中和活性鉴定。结果:IL-17疫苗(0002)免疫新西兰兔产生的抗体能IL-l7特异地结合,并且能够有效地阻断人IL-17刺激HT1080细胞产生IL-6。结论:所制备的IL-17疫苗(0002)所产生的抗体具有高度的特异性、稳定性及中和活性,为针对IL-17为靶点的药物的开发奠定了基础。

Objective To IL-17 vaccine design,to immunize New Zealand rabbits, production and purification of antibodies to IL-17,by combining the rate of detection of antibodies and inhibition of IL-17 function rate,to select a kind of function of IL-17 had high inhibition rate of vaccine.Methods The screening design 4 IL-17 peptide（0001,0002,0003,0004）,the vaccine to immunize New Zealand rabbits obtained with 4 IL-17 design of the peptide coupling,produce antibodies to different peptides,affinity chromatography method for separation and purification of immunity,anti IL-17 antibody and IL-17 antibody test;combined with ELISA detection,and identification of neutralizing activity of antibody.Results（0002） IL-17 vaccine to immunize New Zealand rabbits produced antibodies specifically binding to IL-l7,and can effectively block the IL-17 stimulates the production of IL-6 HT1080 cells.Conclusion The preparation of IL-17 vaccine（0002） antibody produced with specificity,stability and neutralizing activity of height,for IL-17,which lays the foundation for the development of drug targets.