高速逆流色谱法分离绿茶中的化学成分

Application of High-Speed Countercurrent Chromatography in Purification of Chemical Constituents from Green Tea

采用高速逆流色谱(high-speed countercurrent chromatography,HSCCC)法对绿茶化学成分进行分离。通过对绿茶有效部位的分段萃取和多种溶剂系统的筛选,建立了绿茶化学成分HSCCC分离的方法。经HSCCC分离得到咖啡碱、表没食子儿茶素、表儿茶素、儿茶素、表没食子儿茶素没食子酸酯、没食子儿茶素没食子酸酯、表儿茶素没食子酸酯、没食子酸8种单体化合物,其纯度分别为99.5%、97.2%、98.2%、97.6%、98.8%、99.1%、99.2%和98.8%。该法分离效率高、操作简单,对绿茶在食品医药等领域的应用具有重要意义。

Chemical constituents were separated from green tea by high-speed counter-current chromatography （HSCCC）.Based on stagewise extraction and optimization of solvent systems,the separation of active ingredients by HSCCC was developed.Eight compounds including CFA,EGC,EC,C,EGCG,GCG,ECG and GA with high purity were obtained.The purity of each compound was up to 99.5％,97.2％,98.2％,97.6％,98.8％,99.1％,99.2％ and 98.8％,respectively.The method is of high separation efficiency,simple operation,and great importance for the purification of components from green tea in the food and medical fields.