同步辐射核共振振动能谱学(下)：应用篇

Synchrotron radiation based nuclear resonant vibrational spectroscopy:Applications

文章上篇介绍了核共振振动能谱学的基础理论和实验方法。本篇将着重介绍其在铁基化学和铁基生物化学研究中的应用实例:首先讨论单铁玉红氧还蛋白溶液样品和晶体样品的核振能谱以及它们的对比情况;然后由简入繁,系统地叙述核振能谱学方法在铁硫蛋白、氢化酶、固氮酶等一系列重要的以铁硫为基础的多铁蛋白研究中的应用和相关的研究成果;作为非铁硫类蛋白的代表,作者也简述了一氧化碳肌红蛋白的核振能谱。以上述实例为基础,本篇最后综述了该能谱方法在理论上和实验上突出的优越性和它在国内外的发展前景。

In the article in the last issue （Part 1）, we discussed the theoretical concept and experimental aspects of nuclear resonant vibrational spectroscopy （NRVS）. In this issue, we will fo-cus on its practical applications in iron-specific chemistry and biochemistry. As an introduction, the NRVS techniques for solution and crystal samples of single iron rubredoxin are first described and compared. Then the recent research results of multi-iron iron-sulfur proteins such as ferredoxins, hydrogenases and nitrogenases are discussed in detail. As an example of non-iron-sulfur proteins, carbon monoxide bound myoglobin is also presented. Based on the above examples, the theoretical and experimental advantages and potential of NRVS are reviewed, and its future prospects at home and abroad are discussed.