水稻白条纹叶突变体st11的遗传分析与基因定位

Genetic Analysis and Gene Mapping of White Stripe Leaf Mutant st11in Rice

白条纹叶突变体st11是从粳稻品种Kitaake组培过程中获得的。该突变体在分蘖前叶色表现为正常,从分蘖期开始新生叶表现为白条纹直至成熟期。与野生型相比,该突变体的分蘖、株高、结实率和千粒重等农艺性状没有发生明显变化。遗传分析表明该突变体白条纹叶性状受一对隐性核基因控制。利用该突变体分别与水稻02428、Jodan杂交构建了两个F2群体用于基因定位。通过集群分离分析(bulked segregant analysis)发现该基因位于第1染色体端粒附近,并与分子标记RM151和RM10080连锁。进一步利用更多分子标记分析F2群体,我们将该基因定位于I10和I26两个标记之间大约270kb的区间内。

A white stripe leaf mutant st1 1 was obtained from tissue-cultured rice Kitaake （Oryza sativa spp j aponica ）. This mutant was characterized by white stripe leaves from tillering stage to mature stage.Compared with the wild type Kitaake,the mutant showed no obvious changes in agricultural traits,such as tiller number,plant height,seed-setting rate,and grain weight.Genetic analyses showed that the white stripe leaf phenotype in the mutant st1 1 was controlled by a single recessive gene.Two F2 segregating populations derived from the crosses,st1 1×Jodan and st1 1 ＆#215;02428, respectively,were used for mapping the gene st1 1 .Bulked segregant analysis suggested that the gene st1 1 was located close to the markers,RM1 5 1 and RM10080,on chromosome 1 .Using more molecular markers,we finally delimited the gene st1 1 to a region of about 270 kb between the Indel markers,I10 and I26,on the telomere of the short arm of chromosome 1 .