老鹳草素对地塞米松诱发大鼠骨质疏松症的影响

Effect of geraniin on dexamethasone-induced rat osteoporosis

目的 观察老鹳草素对地塞米松诱发大鼠骨质疏松症的影响.方法 将6个月龄雌性Wistar大鼠随机分为对照组、模型组、5、10、20 mg/kg老鹳草素组和2 mg/kg阿仑膦酸钠组.大鼠肌肉注射地塞米松（1 mg/kg）,老鹳草素连续灌胃90d,对股骨和腰椎进行骨密度及骨组织形态计量学检测.结果 对照组股骨和椎骨骨密度分别为（0.20±0.01）和（0.18±0.01） g/cm^2,模型组分别为（0.15±0.01）和（0.10 ±0.01） g/cm^2,差异有统计学意义（P＜0.05）.与模型组比较,10、20 mg/kg老鹳草素能显著提高股骨和椎骨的骨密度.5 mg/kg老鹳草素可增加模型组大鼠股骨骨小梁表面积百分率、骨小梁间距和骨小梁节点数;10、20 mg/kg老鹳草素明显增加模型组大鼠股骨和椎骨骨小梁计数、骨小梁宽度和骨小梁表面积百分率,增多小梁节点数、减少骨小梁间隙距离和小梁游离末端数.结论老鹳草素具有抑制地塞米松引起大鼠骨质疏松症的作用,并呈剂量-效应关系.

Objective To observe the effect of geraniin on dexamethasone-induced experimental osteoporosis.Methods The female Wistar rats were randomly divided into control group,model group,geraniin （5,10 or 20 mg/kg） groups and alendronate group.The bone mineral density （BMD） and histomorphometry of both femur and lumbar vertebra were measured.Results The BMD of the femur and lumbar vertebra in the control group was （0.20 ±0.01） and （0.18 ±0.01） g/cm^2,and that in model group was （0.15 ±0.01） and （0.10 ±0.01） g/cm^2,with the difference being significant （P 〈0.05）.As compared with the model group,10 and 20 mg/kg geraniin significantly increased the BMD of both femur and lumbar vertebra.For the model rats administrated with geraniin （5 mg/kg）,some parameters including percentage of trabecular area （% Th.Ar）,the trabecular separation （Tb.Sp） and trabecular joint （JOINT） were increased.For both fermur and vertebra,geraniin （10 or 20 mg/kg） increased the trabecular number （Th.N）,trabecular width （Tb.Wi）,% Tb.Ar and JOINT of the model rats.Conclusion Geraniin inhibits dexamethasone-induced rat osteoporosis with a dose-response relationship.