摘要
目的 检测甲状腺肿瘤患者外周血及肿瘤组织中辅助性T细胞22 (Th22)及白细胞介素(IL)-22的表达水平,探讨其与患者临床病理特征的关系.方法 选择56例原发甲状腺肿瘤患者和30例健康志愿者,采用流式细胞仪检测外周血Th22细胞水平,酶联免疫吸附试验法(ELISA)检测血清中IL-22表达水平,应用免疫组织化学双染色分析正常组织及肿瘤组织中Th22阳性细胞的浸润程度,分析这些参数与临床病理特征间的关系.结果 甲状腺肿瘤患者外周血中Th22细胞及IL-22水平分别为(1.74±0.27)%和(162.7±32.2) ng/L,显著高于对照组[(0.57±0.14)%,t=15.72,P<0.01;(64.2 ±20.7) ng/L,t=11.31,P<0.01];甲状腺癌患者的Th22细胞比例及IL-22水平明显高于甲状腺瘤患者[(2.21±0.34)%比(1.39±0.22)%,t=9.14,P<0.01;(199.1±34.1)ng/L比(129.8±31.3) ng/L,t=6.79,P<0.01].甲状腺肿瘤患者组织中Th22阳性细胞浸润率为56.25%,而对应正常组织为8.33%,差异有统计学意义(χ^2=8.167,P<0.01);甲状腺癌组织中Th22细胞浸润率为78.6%,甲状腺瘤组织为38.9%,差异有统计学意义(χ^2=5.039,P<0.05).外周血Th22细胞比例、IL-22的表达水平和肿瘤组织中Th22阳性细胞浸润程度与临床分期、淋巴结转明显相关(P<0.05),与年龄、性别、病理类型无明显相关(P>0.05).结论 甲状腺肿瘤患者外周血Th22细胞比例及血浆中IL-22水平显著升高,且与临床分期等病理特征明显相关,表明Th22细胞及IL-22参与甲状腺肿瘤的发生发展.
Objective To detect the expression of T helper cells 22 (Th22) in the peripheral blood and the level of interleukin (IL)-22 in the plasma and the Th22 cells in the tumor tissues of patients with thyroid tumor,and to analyze the relationship between Th22/IL-22 and clinical characteristics in the pathogenesis of laryngeal squamous cell carcinoma (LSCC).Methods Fifty-six patients with thyroid tumor,and 30 healthy controls were included.Th22 cells in the peripheral blood were detected by flow cytometry,the concentrations of IL-22 in serum were measured by enzyme-linked immunosorbent assay (ELISA),Double immunohistochemistry (IHC) was used to determine the Th22 cells in paraffin-embedded tissues,the relationship between Th22/IL-22 and clinical characteristics were analyzed.Results The level of Th22 cells in peripheral blood and IL-22 in the serum of patients with thyroid tumor were (1.74 ± 0.27) % and (162.7 ± 32.2) ng/L,respectively,significantly higher than that of healthy controls [(0.57±0.14)%,t=15.72,P〈0.01; (64.2±20.7) ng/L,t=ll.31,P〈0.01].Patients with thyroid carcinomas had higher Th22 cells and IL-22 levels than that of patients with thyroid adenomes [(2.21±0.34)% vs.(1.39±0.22)%,t=9.14,P〈0.01; (199.1 ±34.1) ng/Lvs.(129.8±31.3) ng/L,t =6.79,P 〈0.01].Compared with normal tissues,tumor tissues showed higher expression of CD4 + IL-22 + cells (56.25 % vs.8.33%,χ^2 =8.167,P 〈 0.01).Compared with adenome tissues,carcinoma tissues showed higher expression of CD4 + IL-22 + cells (78.6% vs.38.9%,χ^2 =5.039,P 〈0.05).The levels of Th22 cells in peripheral blood,IL-22 in serum and Th22 cells in tumor tissues was associated with tumor stage and lymph node metastasis (P 〈 0.05).Conclusion There are higher quantification of Th22 cells and IL-22 in the peripheral blood and tumor tissues in patients with thyroid tumor and they have positive correlation with the clinical stage,Th22 cells and IL-22 may contribute to the disease progression and prognosis of thyroid tumor.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2015年第1期147-150,共4页
Chinese Journal of Experimental Surgery