微小RNA-21在乳腺癌患者血清中的表达及其与临床病理特征的关系

Expression of serum microRNA-21 in breast cancer patients abd its association with clinicopathological features

目的 观察血清微小RNA（miRNA,miR）-214、miR-21和miR-195在乳腺癌患者和健康人血清中的表达,探讨这3个miRNA和乳腺癌的相关性.方法 收集40例乳腺癌患者和10例健康女性的血清,从血清中提取总RNA,应用实时荧光定量聚合酶链反应（FQ-PCR）技术检测这3个miRNA在两类人群中的表达.应用SPSS 21.0软件对血清中miRNAs的表达水平的差异采用Mann-Whitney U检验进行分析.结果 miR-21在乳腺癌患者血清中的表达量为0.120（0.043～0.299）和正常对照组血清中的表达量[0.011 （0.003 ～0.063）]比较显著上调（P＜0.01）;且血清中miR-21与乳腺癌临床分期、淋巴结转移相关（P＜0.01）.结论 血清miR-21和乳腺癌的临床病理特征明显相关.

Objective To study the expression of serum microRNA （miRNA,miR）-214,miR-21 and miR-195 in the breast cancer patients and healthy people,and its correlation to the clinicopathological features of the patients.Methods We collected the serum samples from 40 cases of breast cancer,and the serum samples from 10 healthy women as the controls.The total RNA was extracted from the samples for quantitative analysis of miR-214,miR-21 and miR-195 using real-time fluorescent quantitative polymerase chain reaction （FQ-PCR）.Results The serum levels of miR-21 expression in the breast cancer patients and healthy women are 0.120 （0.043-0.299） and 0.011 （0.003-0.063）,the serum levels of miR-21 expression were significantly higher in the cancer patients than in the healthy control group （P 〈0.01） ; In the cancer patients,high miR-21 expression was significantly correlated to advanced clinical stage （P 〈 0.01） and lymph node metastasis （P 〈 0.01）.Conclusion Serum miR-21 has a significant correlation with the clinicopathological features of breast cancer.