补肺通络方不同时期给药对内毒素小鼠肺纤维化的治疗作用

Rolesof invigorating lung and promotingmeridians formula at different periods of administration in treatment of endotoxin-induced pulmonary fibrosis in mice

目的:观察补肺通络方不同时期给药对内毒素小鼠肺纤维化的治疗作用。方法:内毒素腹腔注射制备小鼠肺纤维化模型,单纯模型组(A组)造模后当日起给予等体积生理盐水,即刻给药组(B组)当日起给予补肺通络治疗,延迟给药组(C组)第11天起给予补肺通络治疗。第28天处死所有小鼠,HE染色观察肺形态学改变,Masson染色观察肺纤维化程度。结果:肺损伤评分A组(0.82±0.14)与B组(0.49±0.14)(P<0.01)和C组(0.34±0.08)(P<0.01)比较,B组与C组(P<0.05)比较差异具有统计学意义。(2)纤维化评分C组(14.25±2.25)低于A组(18.44±2.65)(P<0.01)和B组(17.71±3.25)(P<0.05),A组和B组相比差异无统计学意义。结论:补肺通络疗法对内毒素小鼠肺纤维化具有明确治疗作用,但应避免在急性炎症阶段给药。

Objective To observe the roles of invigorating lung and promoting meridians （ILPM） formula at different periods of administration in the treatment of endotoxin-induced puhnonary fibrosis in mice. Methods A mouse model of pulmonary fibrosis was established by intraperitoneal injections ofendotoxin.The control group （group A） was given normal saline on the day of endotoxin injection （d0）; immediate administration group （group B） received ILPM on d0, while delayed administrated group （group C） received ILPM on dll. All the mice were sacrificed on d28. HE staining was used to observe the morphological changes of the lungs. Masson staining was applied to detect the degree of fibrosis. Results There were statistical significance in scores for lung injury between group A and group B [（0.82±0.14） vs. （0.49±0.14）, P〈0.01], group A and group C [ （0.82±0.14） vs. （0.34±0.08）, P〈0.01 ）], and group B and group C （P〈0.05）. The scores for fibrosis differed statistically between group Cand group A [（14.25±2.25） vs. （18.44±2.65）, P〈0.01], and between group C and group B [（14.25±2.25） vs. （17.71±3.25）,P〈0.05]. Conclusions Invigorating lung and promoting meridiansformula plays a definite role in the treatment ofendotoxin-induced pulmonary fibrosis, but theformula should not beadministrated in the acute phase of lung injury.