摘要
应用超高效液相色谱－串联质谱技术建立了兽用中药制剂穿心莲注射液和鱼腥草注射液中非法添加庆大霉素的检测方法。样品经水溶解后稀释，采用20 mmol／L七氟丁酸（ HFBA）乙腈与20 mmol／L HFBA水溶液作为流动相进行梯度洗脱，经ACQUITY HSS PFP色谱柱分离，通过多反应监测模式进行测定。庆大霉素在0．02～2μg／mL的范围内线性关系良好，相关系数0．9998。平均回收率为98．2％～100．3％，相对标准偏差小于2．4％。检测限为2 mg／mL，定量限为4 mg／mL，该法准确、简单、快速。
A method of Ultra-HPLC-MS/MS was developed to determine gentamycin illegally added in traditional Chinese medicine for animals. The samples were dissolved and diluted. The HPLC separation was performed on ACQUITY HSS PFP column with a linear gradient elution program of 20 mmol/L HFBA acetonitrile and 20 mmol/L HFBA aqueous solution as the mobile phase. Selective reaction monitoring was used for the selective detection. The linearity of gentamycin ranged from 0.02 to 2μg/mL with coefficient 0.9998. The average recovery was within the range of 98.2%~100.3% and RSD were less than 2.4%. The limit of detection was 2 mg/mL and limit of quantification was 4 mg/mL. The method was accurate,convenient and fast.
出处
《中国兽药杂志》
北大核心
2015年第1期41-44,共4页
Chinese Journal of Veterinary Drug
