膜联蛋白Ⅱ在乳腺癌组织中的表达以及对乳腺癌细胞侵袭的影响

Expression of Annexin Ⅱ in breast cancer and the effects on the invasion of breast cancer cells

目的探讨膜联蛋白Ⅱ在乳腺癌中的表达以及对乳腺癌细胞侵袭的影响。方法分别采用免疫组化方法检测AnnexinⅡ蛋白在正常乳腺组织、导管原位癌以及侵袭性导管癌组织中的表达情况,并测定高侵袭性乳腺癌细胞株及低侵袭性乳腺癌细胞株中AnnexinⅡm RNA(RT-PCR法)以及蛋白(蛋白印记技术)的表达情况。结果 AnnexinⅡ在正常乳腺组织中主要表达在间质内,在乳腺导管上不表达,在导管原位癌以及侵袭性导管癌组织中间质、血管、肿瘤组织上均有表达。AnnexinⅡ在正常乳腺组织表达的IOD值为(6.5±1.0)×106,在导管原位癌中表达的IOD值为(27.5±3.0)×106,在侵袭性导管癌组织中表达的IOD值为(40.0±11.0)×106。三组间比较,差异有高度统计学意义(P<0.01),导管原位癌、侵袭性导管癌组织中的表达量显著高于正常乳腺组织,而侵袭性导管癌中的表达量显著高于导管原位癌(均P<0.01)。AnnexinⅡm RNA在高侵袭性人乳腺癌细胞株MDA-MB-435s中的表达灰度值(0.83±0.11)显著高于MCF-7株中的表达(0.07±0.01),差异有高度统计学意义(P<0.01);AnnexinⅡ蛋白在高侵袭性人乳腺癌细胞株MDA-MB-435s中的表达(1.47±0.08)显著高于MCF-7株中的表达(0.11±0.03),差异有高度统计学意义(P<0.01)。结论 AnnexinⅡ在乳腺癌中高表达,并且瘤细胞侵袭性越强,其表达也越强。

Objective To discuss expression of Annexin II in breast cancer and the effects on the invasion of breast cancer cells. Methods Annexin II expression in normal breast tissue, ductal carcinoma in situ and invasive ductal carcinoma tissues were detected by immunohistochemical method, and Annexin II mRNA （RT-PCR） and protein （Western blot） expressions in high invasive breast cancer cell lines and low invasive breast cancer cell lines were detected. Results In normal breast tissue, Annexin II was mainly detected in troma, without expression on breast catheter. In ductal carcinoma in situ and invasive ductal carcinoma tissues, Annexin II was detected in troma, vessel and tumor tissue. IOD of Annexin II expression in normal breast tissue was （6.5±1.0）×10^6, in ductal carcinoma was （27.5±3.0）×10^6, and in invasive ductal carcinoma tissues was （40.0±11.0）×106. There was significant difference in three groups （P＆lt;0.01）. IOD of AnnexinII expression in invasive ductal carcinoma tissues and ductal carcinoma was higher than that in normal breast tissue, and IOD of Annexin II expression in invasive ductal carcinoma tissues was higher than that in ductal carcinoma （all P＆lt;0.01）. Gray value of AnnexinII mRNA expression in high invasive breast cancer cell lines MDA-MB-435s was （0.83±0.11）, which was higher than that in low invasive breast cancer cell lines MCF-7 （0.07±0.01）, and there was significant difference between two cell lines （P＆lt;0.01）. IOD of AnnexinIIprotein expression in high invasive breast cancer cell lines MDA-MB-435s was （1.47±0.08）, which was higher than that in low invasive breast cancer cell lines MCF-7 （0.11±0.03）, and there was significant difference between two cell lines （P＆lt;0.01）. Conclusion AnnexinII shows high expression in breast cancer, and the stronger aggressive of tumor cells is, the higher expression of it is.