胆囊癌中Ki67和MMP-2的表达及临床意义

Expression of Ki67 and MMP-2 in gallbladder carcinoma and its clinical significance

目的检测Ki67抗原和基质金属蛋白酶(MMP-2)在胆囊癌及慢性胆囊炎组织中的表达,探讨两者与胆囊癌的增殖、侵润及转移的关系。方法应用免疫组化SP法检测Ki67和MMP-2在52例胆囊癌患者的癌组织及21例慢性胆囊炎患者的胆囊组织中的表达情况。结果 Ki67和MMP-2在胆囊癌组织中阳性表达率分别为84.6%和78.8%;在慢性胆囊炎胆囊组织中的阳性表达率分别为42.9%和38.1%,差异均有统计学意义(P<0.05)。Ki67的阳性表达与胆囊癌的分化程度、Nevin分期有关(P<0.05),MMP-2的阳性表达与胆囊癌的分化程度、Nevin分期及淋巴结转移有关(P<0.05)。两者均与患者的年龄、性别及肿瘤的大小无相关性(P>0.05)。Ki67与MMP-2在胆囊癌组织中的阳性表达呈正相关(r=0.427,P=0.002)。Ki67和MMP-2表达阴性的患者术后生存期高于表达阳性的患者。结论 Ki67和MMP-2的阳性表达与胆囊癌的增殖和浸润有关,联合检测Ki67和MMP-2,在一定程度上有助于判断胆囊癌进展及预后。

Objective To investigate the expressions of Ki67 antigen and matrix metallo protinase-2 （MMP-2） in the gallbladder carcinoma and chronic cholecystitis tissue,and to analyze their correlation with proliferation,invasion,metastasis of gallbladder carcinoma.Methods SP immunohistochemistry was used to determine the expression of Ki67 and MMP-2 in 52 gallbladder carcinomas,21 chronic cholecystitistissue.Results The positive rate of Ki67 and MMP-2 in gallbladder carcinomas are respectively 84.6％,78.8％.significandy higher than chronic cholecystitis （Ki67：42.9％,MMP-2：38.1％,P ＜ 0.05）.The expressions of Ki67 were correlated with the tumor differentiation,Nevin stages （P ＜ 0.05）,and the expressions of MMP-2 were correlated with the tumor differentiation,Nevin stages and Lymph node Metastasis （P ＜ 0.05）,both of them are not with the patient,s age,gender and tumor size （P ＞ 0.05）.Ki67 antigen expression was positively correlated with matrix metallo protinase-2 （MMP-2） expression in gallbladder carcinoma（r =0.427,P =0.002）.Patients with negative expression of Ki67 and MMP-2 had longer survival time than those with positive expression of Ki67 and MMP-2.Conclusion The expression of Ki67 and MMP-2 was associated with proliferation,invasion,metastasis of gallbladder carcinoma,combined detection of Ki67 and MMP-2 is conducive to judging the progress and prognosis of the gallbladder carcinoma.