米糠脂溶性单体化合物的分离与结构鉴定

Isolation and Identification of Lipid-Soluble Compounds from Rice Bran

以米糠为原料,采用有机溶剂提取得到粗提物,利用硅胶柱层析和Sephadex LH-20凝胶柱层析等分离方法从米糠粗提物中分离得到9个单体化合物,通过核磁共振(nuclear magnetic resonance,NMR)和电喷雾质谱(electrospray ionization mass spectrometry,ESI-MS)鉴定其化学结构分别为油酸、亚油酸、2,3-二羟基硬脂酸丙酯、2,3-二羟基油酸丙酯、3-羟基1,2-二油酸丙二酯、1,3-二油酸丙二酯、1,3-二油酸-2-硬脂酸甘油酯、豆甾醇、麦角甾醇。

Crude extract was obtained by the extraction of rice bran using organic solvent. Nine pure compounds were isolated and identified from the extract using silica gel column chromatography and Sephadex LH-20 column chromatography. Their chemical structures were elucidated as oleic acid, linoleic acid, 2,3-dihydroxypropyl stearate, 2,3-dihydroxypropyl oleate, 3-hydroxy-l,2-propyl diolein, l,3-diolein, 1,3-2-oleyl,2-stearoyl triacylglycerol, stigmasterol, and ergosterol by nuclear magnetic resonance （NMR） and electro-spray ionization mass spectrometry （ESI-MS）.