浓缩苹果汁生产过程中cAMP分布规律研究

Distribution of cAMP during Production of Concentrated Apple Juice

探究浓缩苹果汁生产过程中3’,5’-环磷酸腺苷(3’,5’-cyclic adenosine monophosphate,cAMP)含量的分布规律。利用HPLC方法测定水浸提和乙醇浸提两种不同方式处理榨汁后的苹果果渣的cAMP含量和浓缩果汁中cAMP的含量,分析不同稀释倍数、不同可溶性固形物含量的浓缩苹果汁cAMP变化规律,并利用cAMP分配系数(DCcAMP)描述榨汁过程中cAMP含量的分布规律。结果表明:榨汁后苹果果渣中cAMP含量为(63.444 1±2.323 6)?g/g,70°Brix的浓缩苹果汁成品中cAMP含量为(58.611 3±1.349 3)?g/mL,30°Brix的浓缩苹果汁半成品中cAMP含量为(12.018 3±0.583 9)?g/mL;建立了浓缩苹果汁中cAMP含量与可溶性固形物含量之间的关系方程为y=0.808 5x-1.218 5;测定得到70°Brix和30°Brix浓缩苹果汁的DCcAMP分别为92%和19%。因此,在浓缩苹果汁生产过程中,cAMP更趋向于存在苹果果渣中,且浓缩工艺对cAMP有富集作用。

The distribution pattern of 3＇,5＇-cyclic adenosine monophosphate（cAMP） during the production of concentrated apple juice was investigated. HPLC was employed to determine the cAMP content in apple pomace left after juice extraction with water or aqueous ethanol and in concentrated apple juice. The change trend of cAMP content in concentrated apple juice was analyzed as a function of dilution factor and soluble solid content. Distribution coefficient （DCcAMP） was calculated to describe the distribution regularity of cAMP. The result showed that cAMP contents in apple pomace, 70 ° Brix concentrated apple juice, and 30 ° Brix concentrated apple juice were （63.444 1 ± 2.323 6） tag/g, （58.611 3 ± 1.349 3） μg/mL and （12.018 3± 0.583 9） μmL, respectively. An equation indicating cAMP content （y） as a function of soluble solid content （x） in concentrated apple juice was established as follows： y = 0.808 5x - 1.218 5. Furthermore, the DCcAMP of 70 ° Brix and 30 ° Brix concentrated apple juice were 92% and 19%, respectively. Thus, during the production of concentrated apple juice, cAMP preferred to exist in apple pomace and could be enriched by the concentration process