金褐霉素高产相关基因的筛选与表达

Screening and Expression of Regulatory Gene for Enhanced Aureofuscin Production

通过高效表达金褐霉素生物合成基因簇中的调控基因来提高其产量。本实验从野生金褐链霉菌SYAU0709克隆aurJ3M基因,连接载体pMD19-T并测序。将带有aurJ3M基因的表达质粒pBJAUM转化金褐链霉菌SYAU0709,高效表达aurJ3M基因,获得重组菌株。通过高效液相色谱分析检测,重组菌株的金褐霉素产量提高约6倍,并且遗传性能稳定,发酵结果说明过量表达aurJ3M基因能够提高金褐霉素的产量。

Aims： To our knowledge, the production of aureofuscin is very low in the wild-type strain of Streptomyces aureofuscus （S. aureofuscus）. This study attempted to increase the production of aureofuscin by over-expression of a regulatory gene in the wild-type strain. Methods and Results： The aurj3M gene was amplified by PCR from S. aureofuscus SYAU0709, ligated into the vector pMD19, and sequenced. Recombinant bacterial strains were constructed by transforming SYAU0709 with an expression plasmid （pBJAUM） that contained aurj3M, thereby increasing the number of aurj3M gene copies. Conclusions： The fermentation results showed aurJ3M gene could promote aureofuscin production. Specifically, the recombinant strain produced approximately 600% more aureofuscin, as quantified by high-performance liquid chromatography analysis. The recombinant strain also had good genetic stability.