五倍子单宁脂质体的制备及质量评价

Preparation of gallnut tannins liposome and its quality evaluation

以超声波辅助溶剂提取法提取五倍子单宁,逆相蒸发法制备五倍子单宁脂质体,研究制备脂质体最佳工艺,并对在优化条件下制备的脂质体进行理化性质评价。结果表明优化条件为:卵磷脂与胆固醇的质量比(SPC/CH)为2∶1、有机相与水相体积比(O/A)为6∶1、缓冲液p H6.5。在此条件下质量浓度为1mg/m L的五倍子单宁溶液制备的五倍子单宁脂质体包封率为72.26%。结构描述:有效粒径为(79.39±11.62)nm,Zeta电位为-44.7m V,稳定性:4℃条件下储存稳定性良好。逆相蒸发法制备脂质体工艺简单可行,包封率高,粒度均匀,稳定性好,可用于五倍子单宁脂质体的制备。

Ultrasonic solvent extraction was used to eatarct the tannins in gallnut,and reverse-phase evaluation was used to prepare liposomes of gallnut tannins.The best technology was obtained and its physical and chemical properties were evaluated.The results showed that when the SPC/CH was 2： 1,the O/A was 6：1, and the pH was 6.5,the entrapment efficiency of liposomes prepared by gallnut tannins was 72.26%,the effective diameter was （79.39 ±11.62）nm and the zeta potential was-44.7 mY,the favorable storage temperature was 4℃. Preparation process of liposome using reverse-phase evaporation was simple and feasible, high encapsulation efficiency, particle size uniformity,stability and can be used for preparation of gallnut tannins liposome.