杜仲雄花茶多糖的响应面优化提取及其抗氧化活性评价

Study on the optimization of extracting polysaccharides from Eucommia male flowers tea by response surface methodology and the antioxidant evaluation

为了寻找杜仲雄花茶多糖的提取工艺,考察多糖的抗氧化活性,采用Design Expert 8.0软件设计实验,并用响应面法优化提取工艺,以DPPH·清除率、·OH清除率和还原力等指标评价杜仲雄花茶多糖的抗氧化活性。结果表明:杜仲雄花茶多糖的提取工艺参数为:提取温度90℃、提取时间4.5h、液料比15∶1。在此工艺条件下,多糖提取得率为3.48%。以合成抗氧化剂BHT为对照,1mg/m L杜仲雄花茶多糖对DPPH·的清除率为52.5%,还原力为72.73%,对·OH的清除率为63.1%。杜仲雄花茶具有一定的抗氧化活性。

The optimum extraction process of polysaccharides from Eucommia male flowers tea and its antioxidant activity were explored. The process was designed by Design Expert 8.0, and the extraction conditions were optimized by response surface methodology（ RSM）.The antioxidant activities were examined from the aspects of scavenging rate of DPPH and hydroxyl free radicals, reducing power, respectively.The results indicated that the optimum extraction conditions were： extraction temperature 90℃, extraction time 4.5h, ratio of solvent to material 15：1. Under these conditions,the yield of polysaccharides had 3.48%.Compared with BHT,the rate of scavenging DPPH radicals was 52.5% ,the reducing power reached to 72.73% ,and the rate of scavenging hydroxyl radical was 63.1% when the Eucommia male flower polysaccharide concentration was 1mg/mhThe above results indicated that the Eucommia male flower polysaccharides had antioxidant effects in vitro.