摘要
不同的水稻品种对外界低温胁迫的反应不同,研究水稻的耐冷机制对水稻生产至关重要。本研究选取云南栽培的楚粳26为材料,以常温[28℃(06:00—18:00)/25℃(18:00—06:00)]为对照,采用两种不同的昼夜低温处理,即:处理1[20℃(06:00—18:00)/12℃(18:00—06:00)]和处理2[18℃(06:00—18:00)/5℃(18:00—06:00)],探究该品种对不同外界处理处理的生理生化反应机制。研究表明:处理1中,谷胱甘肽还原酶(GR)活性迅速增高,其他抗氧化酶未出现较大变化;处理2中,过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性均出现一定程度的升高。花粉育性检测证实,处理2较处理1对花粉影响更大;同时,处理2较处理1对叶绿素荧光参数Fv/Fm的影响也更大,但都未对光合系统造成深度的伤害(>0.82)。结果证实:楚粳26对低温具有一定的耐受性,且抗氧化酶系统对抗低温具有重要的作用。
Different rice varieties have different reactions to temperature stress, so it is very important to understand the cold resistant mechanism. We took the rice variety "Chujing-26" as test materials to study the physiological and biochemical effect under two different low temperature treatments [ treatment 1:(20 ℃ (06: 00-18: 00) / 12 ℃ (18: 00-06: 00), treatment 2:(18 ℃) (06: 00- 18: 00) /5℃(18:00-06: 00)], using normal temperature [28 ℃ (06: 00-18: 00) /25 ℃ ( 18 : 00-06: 00) ] as control. The result showed that glutathione reductase (GR) activity increased greatly under treatment 1 while other enzymes activity showed little change. Under treatment 2, the activity including peroxidase (POD), aseorbate peroxidase (APX), catalase (CAT), superoxide dismutase (SOD) increased more than control. The result of pollen fertility test showed that treatment 2 was more severe than treatment 1. At the same time, determination of chlorophyll fluorescence parameters of Fv/Fm also supported that treatment 2 was more severe than treatment 1, but neither treatment destroyed rice photosynthetic apparatus. The experimental resuhs supported the adaptability of Chujing-26 to low temperature change, and the antioxidant enzyme activities play an important role.
出处
《云南农业大学学报(自然科学版)》
CAS
CSCD
北大核心
2015年第1期25-29,共5页
Journal of Yunnan Agricultural University:Natural Science
基金
国家"973"计划资助项目(201CB100400)
关键词
耐冷性水稻
低温
抗氧化酶活性
孕穗期
cold resistance of rice
low temperature
antioxidant enzyme activity
booting stage