无籽刺梨离体快繁技术研究

Study on in vitro Propagation of Rosa sterilis

采用无籽刺梨嫩枝茎段为外植体,MS为基本培养基,进行无籽刺梨组织培养研究,探讨了影响无籽刺梨组培快繁的主要因素,包括外植体的消毒;最佳激素浓度及组合;最佳生根培养基及组培苗的移栽等。结果表明:无籽刺梨茎段在0.1%升汞中处理12min中消毒效果最佳,有效存活率达77.78%;腋芽诱导的最佳激素配比为6-BA0.5mg/L+IBA0.1mg/L,诱导率为100%;去顶后芽的增殖最适培养基为MS+6-BA0.2mg/L+IBA0.02mg/L,增殖倍数可达3.22倍;在1/2MS+IBA0.3mg/L的生根培养基上,不定芽生根率为100%,移栽后成活率在95%以上。

The young stem was taken as the explants for studying on tissue culture of Rosa sterilis. The factors of tissue culture were studied, including the disinfection of explants, the selection of optimum consistency and combination of hormones, the selection of optimum culture medium combinations of root induction and the transplant of the tissue culture seedling. The results showed that the optimum explants sterilization time was dip in 0. 1% HgCl2 for 12 min, and the effective survival rate was 77.78%. The suitable combination for the inducement of axillary buds was MS ＋ 6-BA 0. 5 mg/L ＋ IBA 0. 1 mg/L, and germination rate was 100%. Shoots cultured on the medium of MS ＋ 6-BA 0. 2 mg/L ＋ IBA 0. 02 mg/L could be multiplicated by 3.22 fold and above. The media formula for rooting was 1/2 MS ＋ IBA 0. 3 mg/L. In this media, the rootage rate was 100%, and the survival rate was 95% after transplanting.