通心络对早期糖尿病肾病大鼠血管内皮生长因子表达的影响

Effects of Tongxinluo on VEGF expression in early diabetic nephropathy rats

目的观察通心络对早期糖尿病肾病大鼠血管内皮生长因子表达的影响。方法采用高脂饲料+小剂量链脲佐菌素建立2型糖尿病大鼠模型,随机分为空白组10只、模型组10只、缬沙坦组10只(10mg/kg/d)和通心络组10只(0.4mg/kg/d);治疗8周后,比较各组大鼠的空腹血糖、尿白蛋白排泄率、内生肌酐清除率及肾重指数,采用酶联免疫吸附试验测定血浆血管内皮生长因子含量,western blot及RT-PCR测定肾组织中血管内皮生长因子及其受体Flt1的表达。结果模型组大鼠空腹血糖、尿白蛋白排泄率、内生肌酐清除率、肾重指数、血浆血管内皮生长因子含量、肾组织血管内皮生长因子及Flt1表达较空白组有不同程度的升高(P<0.05),通心络组除空腹血糖外各指标水平较模型组均显著降低(P<0.05)。结论通心络可以有效改善早期糖尿病肾病大鼠的高滤过状态,抑制尿蛋白的排出,其作用机制与抑制血管内皮生长因子及其受体Flt1的过度表达有关。

Objective To observe the effect of Tongxinluo on vascular endothelial growth factor （ VEGF） expression in rats with early stage of diabetic nephropathy .Methods The rat model of type 2 di-abetes mellitus was induced by injection of low-dose of streptozocin after having high-fat diet for 4 weeks, 40 rats were randomly divided into 4 groups-normal group, diabetic group, valsartan treated group（10mg/kg/d） and Tongxinluo treated group （0.4mg/kg/d）;After 8 weeks, the level of urinary albumin excretion rate （UAER）, creatinine clearance rate （Ccr）,kidney weight/body weight ratio （KW/BW） and plasma VEGF was measured and the renal expression of VEGF and Flt 1 was determined by western blot and real-time PCR.Results The level of UAER, Ccr, KW/BW, VEGF an Flt1 expression in Tongxinluo treated group was lower than it in diabetic group （P〈0.05）,while FBG had no significant difference .Conclusion Tongxinluo can improve glomerular hyperfiltration and albuminuria excretion in rats with early stage of di -abetic nephropathy;The mechanism could be related to inhibition of VEGF and Flt 1 expression .