炮制配伍“三黄泻心汤”9种组方的药效学比较研究

Pharmacodynamical Study on 9 Component Compatibility of Processed Sanhuangxiexin Decoction

目的基于饮片炮制配伍的成分配伍,从抗炎、活血化瘀及清热等药效学的角度,利用大黄生片、熟片和炭片之间化学成分的组成及量比关系的差异进行"三黄泻心汤"9种组方成分配伍研究。方法动物随机分为正常对照组,模型对照组,阳性药阿司匹林组,9个样品组,大鼠给药剂量为0.5 g生药·kg-1体重,小鼠给药剂量为0.9 g生药·kg-1体重。采用皮下肾上腺素加冰浴的方法建立血瘀证模型,检测不同切变率下的全血粘度、血浆粘度、红细胞压积、血小板聚集率,凝血酶时间和凝血酶原时间以及血浆纤维蛋白原含量;采用皮下注射酵母引起大鼠发热模型,观察给药后0.5、1、2和4 h体温变化;采用二甲苯液致小鼠耳肿胀模型,计算耳肿胀率,观察其抗炎作用。结果组方7可以降低血瘀证模型大鼠的全血粘度和血浆粘度,优于其他组方;组方2、组方4、组方8可以降低小鼠耳肿胀度,且肿胀抑制率高于其他组方,有较好的抗炎消肿效果。结论组方7和8是9种配伍中的优势方。

Objective To compare the pharmacodynamical actions of 9 component compatibility of processed Sanhuangxiexin decoction in terms of anti-inflammation, removing blood stasis and antipyretic effect, and the relationship between the composition and quantity ratio of chemical components from crude Raubarb, prepared Rhubarb and Rhubarb charcoal piece, based on the chemical composition compatibility of processed herbal pieces. Methods Rats were randomly divided into the following groups： control group, model group, positive medicine group（aspirin 0.2 g.kg^-1） and 9 component compatibility groups. The dose for the rat was 0.5 g.kg^-1 and 0.9 g.kg^-1 for the mouse. All groups were treated continuously for 7 days. The acute blood stasis syndrome model was established by hypodermic injection of adrenalin combined with ice water bath. The indexes of hemorheology including whole blood viscosity, plasma viscosity, packed red cell volume, platelet aggregation and fibrinogen were detected under the different shear rate. The thrombin time and prothrombin time were examined. The mouse ear swelling model induced by dimethylbenzene was used to observe the anti-inflammatory action, the volume of the ear swelling was measured after the modeling, and the degrees of swelling （E%） and inhibiting（I%） were calculated. The rat fever model was made by hypodermic injection of 15% yeast saline, the temperature was measured at 0.5, 1, 2 and 4 h after administration. Results The component compatibility of No.7 group decreased the whole blood viscosity and plasma viscosity more significantly, and the obvious decrease of ear swelling rates was found in No. 2, 4 and 8 group when compared to other component compatibility groups. Conclusion Based on the results, No.7 and 8 group were considered as optimal compatibility of the 9 groups.