HPLC梯度洗脱法同时测定党参养荣丸中的梓醇、毛蕊花糖苷、白术内酯Ⅰ和白术内酯Ⅲ

Determination of Catalpol,Verbascoside,Atractylenolide-I and Atractylenolide- Ⅲ in Dangshen Yangrong Pills by HPLC

目的建立同时测定党参养荣丸中梓醇、毛蕊花糖苷、白术内酯Ⅰ和白术内酯Ⅲ含量测定方法，为完善现有质量标准提供参考。方法依利特HypersilC18色谱柱（4．6mm×200mm，5μm）；以乙腈（A）-0．1％磷酸溶液（B）为流动相，梯度洗脱；体积流量：0．9mL·min-1；检测波长分别为210、210、276、276nm。结果党参养荣丸4个成分梓醇、毛蕊花糖苷、白术内酯Ⅰ和白术内酯Ⅲ进样量分别在0．0586～1．1720μg（r=0．9997）、0．0124-0．2480μg（r=0．9992）、0．0106～0．2120μg（r=0．9994）、0．0172～0．3440gg（r=0．9996）范围内与峰面积呈良好的线性关系，平均加样回收率分别为97．85％、96．71％、97．55％、98．01％，RSD（n=6）分别为1．13％、0．77％、1．22％、1．04％。结论本方法简便、快捷，结果准确，重复性好，可应用于党参养荣丸的质量控制。

Objective To establish a HPLC method for simultaneous determination of catalpol,verbascoside,atractylenolide-Ⅰ and atractylenolide-Ⅲ in Dangshen Yangrong pills. Methods Chromatographic conditions concluded Hypersil C18 （4.6 mm×200 mm,5μm） column with the mobile phase consisting of acetonitrile-0.1% phosphoric acid solution in gradient elution mode at the flow rate of 0.9 mL.min -1.The detection wavelengths were 210,210,276,276 nm respectively. Results Catalpol,verbascoside,atractylenolide-Ⅰ and atractylenolide-Ⅲ in Dangshen Yangrong pills had good linearity in the ranges of 0.0586-1.1720 μg（r=0.9997）,0.0124-0.2480 μg（r=0.9992）,0.0106-0.2120 μg（r=0.9994）,0.0172- 0.3440 μg （r=0.9996） respectively. The average recoveries of four constituents were 97.85%（RSD=1.13%）,96.71% （RSD=0.77%）,97.55%（RSD=1.22%） and 98.01%（RSD=1.04%） respectively. Conclusion This method is simple, accurate, repeatable and can be used for the quality control of Dangshen Yangrong pills.