桔梗总皂苷体外抗解脲脲原体作用实验研究

Study on anti-ureaplasma urealyticum activity of platycodon grandiflorum saponins in vitro

目的 通过MIC、MBC、TIME-KILL曲线及快速杀菌测定,观察PGS在体外对解脲脲原体（Ureaplasma urealyticum,Uu）生长是否具有抑制作用.方法 采用倍比稀释法测定MIC和MBC,利用定量PCR检测不同药物浓度对Uu生长的抑制和快速杀灭作用.结果 PGS体外抗Uu的MIC为16～ 32 μg/mL;MBC为32～ 64μg/mL.16、32μg/mL的PGS对Uu的繁殖具有一定的抑制作用,当浓度达到64 μg/mL时可完全快速抑制Uu的生长繁殖.结论 PGS在体外具有抑制Uu生长繁殖作用.

Objectives To observe the activity of Platycodon grandiflorum saponin （PGS） against Ureaplasma Urealyticum（Uu） through experiments of MIC,MBC,TIME-KILL and rapid bactericidal action.Methods MICs and MBCs were determined by broth microdilution method.The concentration of Uu was detected by quantitative PCR after diferent incubate time.Results MICs were 16 ～ 32μg · mL-1 while MBCs were 32 ～ 64μg · mL-1 for PGS against Uu in vitro.Replication of Uu in PPLO was inhibited by PGS when the concentration was between 16 and 32μg · mL-1.The number of Uu in PPLO broth was reduced and the growth was completely inhibited when the concentration of PGS reached 64μg · mL-1.The growth of Uu was inhibited after mixed with PGS for 1 min in the concentration of 64 μg · mL-1.Conclusions PGS has anti-Ureaplasma Urealyticum activity in vitro,and could inhibite the growth of Uu rapidly.