蚕丝-PLGA网状支架的力学强度检测及其与骨髓间充质干细胞共培养

Detection on mechanical property of mesh-shaped silk-PLGA scaffold and its co-culture with rabbit bone marrow-derived mesenchymal stem cells

目的进行蚕丝-PLGA复合网状支架的力学强度检测及其与骨髓间充质干细胞的体外共培养。方法采用纬编针织法制备蚕丝丝素-PLGA纤维复合网状支架,并进行力学强度测试。在网状支架上滴加Ⅰ型胶原并冷冻干燥,再用纤维连接蛋白表面修饰后,种植兔骨髓间充质干细胞进行体外共培养,观察细胞与支架复合生长情况。结果蚕丝-PLGA网状支架宽约5 mm。支架的最大载荷、抗拉强度、断点伸长率、弹性模量分别为(118.32±11.21)N、(52.90±5.03)MPa、49.79%±5.16%、(177.25±19.18)MPa。支架-MSCs复合物培养2 d的扫描电镜观察显示:MSCs细胞黏附于支架表面呈立体状生长,细胞呈梭形,增殖良好,并分泌基质。结论蚕丝-PLGA复合网状支架具有良好的力学性能与细胞相容性,是一种较有潜力的组织工程韧带的支架材料。

Objective To evaluate mechanical property of a mesh-shaped scaffold knitted by silkworm silk-PLGA and its co-culture with rabbit bone marrow-derived mesenchymal stem cells（ MSCs）. Methods A hybrid mesh-shaped silk-PLGA scaffold was prepared by knitting using a mixture of silkworm silk fibroin fiber and PLGA filament,then filled with collagenⅠ and modified by fibronectin. The biomechanical property of the silk-PLGA scaffold was measured. Then rabbit MSCs were seeded on the surface of the scaffold. The situation of cell growth,matrix formation,as well as the combination of cells and scaffold was observed. Results The width of the scaffold is about 5 mm. The maximum load,tensile strength,breakpoints elongation rates,and elastic modulus of the scaffold was（ 118. 32 ± 11. 21） N,（ 52. 90 ± 5. 03） MPa,49. 79% ± 5. 16%,and（ 177. 25 ±19. 18） MPa,respectively. Scanning electron microscopy observation revealed that rabbit MSCs grew and adhered well. Most of the MSCs showed spindle-shaped with good stretch. Moreover,the MSCs secreted rich cell matrix on the surface of the scaffold. Conclusion The scaffold knitted by silk-PLGA has good biomechanical properties and cytocompatibility. Therefore,it is of great potential for tissue engineered ligament and tendon.