昆虫病原线虫共生细菌对植物寄生线虫卵的毒性研究

Toxicity of Entomopathogenic Nematode Symbiotic Bacteria Strains on Eggs of Plant Parasite Nematodes

为探讨昆虫病原线虫体内携带的共生细菌对植物寄生线虫卵的毒性,从实验室保存的4个品系昆虫病原线虫中分离出4个共生细菌菌株,分别为Photorhabdus luminescens-NJ、Photorhabdus luminescens-Hb（USA）、Xenorhabdus nematophilus-Sc和Xenorhabdus nematophilus-Sc-All,检测了共生细菌对植物寄生线虫卵孵化的影响。结果表明：4个品系共生细菌对大豆胞囊线虫和根结线虫的卵孵化均表现出了明显的抑制作用,对大豆胞囊线虫和根结线虫卵孵化抑制率范围分别为74.1%-100%和57.8%-89.2%,但是不同品系所表现出的抑制作用不同,光杆菌属（Photorhabdus spp.）NJ菌株和Hb（USA）菌株对大豆胞囊线虫卵孵化的毒性明显强于致病杆菌属（Xenorhabdus spp.）Sc和Sc-All菌株,卵孵化抑制率分别为100%和89.7%,而对根结线虫卵孵化的毒性致病杆菌属（Xenorhabdus spp.）SC菌株和SCAll则表现出较好的效果,卵孵化抑制率分别达到89.2%和78.2%。证明了昆虫病原线虫共生细菌对植物寄生线虫卵具有毒性。

Toxicity of entomopathogenic nematodes with symbiotic bacteria on egg hatching of the plant parasitic nematodes was examined. Four strains of entomopathogenic nematode symbiotic bacteria were isolated from different entomopathogenic nematodes including Photorhabdus luminescens-NJ,Photorhabdus luminescens-Hb（ USA）,Xenorhabdus nematophilus-Sc and Xenorhabdus nematophilus-Sc-All. The effect of the four strains on the egg hatching of plant parasitic nematodes was investigated. The result indicated that the four strains significantly inhibited egg hatching of these plant parasitic nematodes. The inhibition range was from 74. 1% to 100% with soybean cyst nematodes and from 57. 8% to 89. 2% with Meloidogyne incognita. But suppressive effects were variable with different strains. The suppression of NJ and Hb（ USA） strains were better than Sc and ScAll strains on soybean cyst nematode,and the percentage of inhibition was 89. 7% and 100% respectively. But strains of Sc and Sc-All suppressed on Meloidogyne incognita better by 78. 2% and 89. 2%. It demonstrated that the symbiotic bacteria was showed toxicity on the plant parasitic nematodes.