野生大豆种质资源对大豆菌核病抗性评价

Identification the Resistance of Wild Soybean Germplasm to Sclerotinia sclerotiorum

采用离体叶柄接种法,利用大豆菌核病分离物jia30和jian29对来自全国11个省份的286份野生大豆资源进行了抗性鉴定。结果表明:在289份材料中,对jia30和jian29均表现抗性的材料有34份,占供试材料的11.8%;抗菌核病菌株jian29的材料50份,占供试材料的17.4%;抗菌核病菌株jia30的材料62份,占供试材料的21.6%。根据野生大豆的来源分析发现,辽宁、黑龙江和吉林存在较多的抗大豆菌核病野生大豆种质资源。

Sclerotinia stem rot of soybean is a fungal disease caused by[Sclerotinia sclerotiorum（ Lib.） de Bary]in the world.Resistance breeding was the most economical and environmentally safe method for controlling the disease,and screening on the resistance germplasm was the base for utilization of resistant cultivars. In this study,286 wild soybean germplasm from 11 provinces in China,were identified for the reaction to strong virulent soybean Sclerotinia isolates of Jia30 and jian29 using leafstalk inoculation in vitro technique. The results showed that there were 34 wild soybean germplasm both resistant to Jia30 and jian29,accounting for 11. 8% of the total materials tested. A total of 50 wild soybean germplasm were resistant to jian29,accounting for 17. 4% of tested materials. 62 materials were disease-resistant to jia30,accounting for 21. 6%. The wild soybean germplasm from Liaoning,Heilongjiang and Jilin with resistance to Sclerotinia was relatively high.