江苏海安地区分离的金黄色葡萄球菌的分子特征及其耐药性分析

Analysis on the molecular characteristics and drug resistance of Staphylococcus aureus isolated from Haian,Jiangsu

目的分析江苏海安地区分离的金黄色葡萄球菌的分子生物学特征及其耐药性。方法收集2011年7月至2012年7月江苏海安地区分离的金黄色葡萄球菌43株,采用生化表型及血浆凝固酶试验鉴定菌株,纸片扩散法和肉汤稀释法检测菌株对12种抗菌药物的敏感性,聚合酶链反应(PCR)检测PV杀白细胞素(PVL)基因,采用多重PCR进行葡萄球菌染色体mec基因盒(SCCmec)分型,运用PCR及序列分析进行金黄色葡萄球菌A蛋白(SPA)基因分型。结果 43株金黄色葡萄球菌中有21株为耐甲氧西林金黄色葡萄球菌(MRSA),t030-Ⅲ为主要流行克隆,该克隆有2个主要的耐药谱型,即P-CX-CN-TOB-E-CC-RA和P-CX-CN-TOBRA。结论江苏海安地区MRSA分离率较高,克隆流行特点明显。

Objective To analyze the molecular characteristics and drug resistance of Staphylococcus aureus isolated from Haian,Jiangsu. Methods A total of 43 isolates of Staphylococcus aureus were collected in Haian from July 2011 to July 2012,and the isolates were identified by biochemical phenotypic test and plasma coagulase test.Antimicrobial susceptibility test to 12 kinds of antibiotics was performed by disk diffusion and broth dilution methods.Panton-Valentine leukocidin( PVL) gene was detected by polymerase chain reaction( PCR),Staphylococcal cassette chromosome mec( SCCmec) typing was conducted according to multiplex PCR,and Staphylococcus protein A( SPA)genotyping was performed by PCR and sequencing. Results A total of 21 isolates of methicillin-resistant Staphylococcus aureus( MRSA) were detected. t030-Ⅲ was the major MRSA clone,including 2 resistant profiles,P-CX-CN-TOB-ECC-RA and P-CX-CN-TOB-RA. Conclusions The isolation rate of MRSA is high,and clone spread is the main reason of resistance prevalence.