江西地方药材野山楂叶质量标准研究

Study on the Quality Standard of Jiangxi Local Herb of Hawthorn Leaves

目的：建立江西地方药材野山楂叶的质量标准.方法：建立药材的性状、显微特征和熊果酸TLC鉴别方法;采用HPLC方法测定熊果酸含量,色谱柱为Thermo BDS HYPERSIL C18（250 mm×4.6mm,5μm）,以甲醇-0.05％磷酸（87∶ 13）为流动相,流速为1.0 mL/min,检测波长为210nm.结果：山楂叶的显微特征性强;熊果酸TLC色谱斑点清晰;熊果酸在0.01984～0.1984mg范围内呈良好线性关系,r=0.9991,回收率为98.71％,RSD为0.64％（n=3）.结论：方法简便,准确,重复性好,适用于江西地方药材野山楂叶的质量控制.

Objective： To establish the quality standard for wild hawthorn leaves. Methods： To establish properties, microscopic characteristics, and ursolic acid TLC identification of traditional Chinese herbs; Content determination of ursolic acid by the method of HPLC, the column was Thermo BDS HYPERSIL C18 （250mm × 4.6mm, 5μm）, using methanol -0.05% phosphate （87：13 ） as mobile phase, the flow rate was 1.0 mL / min, the detection wavelength was 210 nm. Results： The wild hawthorn leaf microstructure had strong characteristics; ursolic acid TLC chromatographic spots were clear; ursolic acid ranged in 0.01984 - 0. 1984 mg showed good linear relationship in the range, the correlation coefficient was 0. 9991. The recovery rate was 98.71% with RSD being 0.64%. （n = 9）. Conclusion： The method is simple, accurate, reproducible, and suitable for the quality control of wild hawthorn leaves.