异源表达酸土脂环酸芽孢杆菌DnaJ基因提高大肠杆菌胁迫抗性

Heterologous Expression of Dna J from Alicyclobacillus acidoterrestris Endowed Escherichia coli with Abiotic Stress Resistance

构建重组质粒p ET28a-Dna J,转化大肠杆菌BL21(DE3),利用IPTG诱导重组大肠杆菌表达酸土脂环酸芽孢杆菌Dna J,对重组大肠杆菌和对照大肠杆菌分别进行热及酸、碱胁迫处理,研究酸土脂环酸芽孢杆菌Dna J基因异源表达对重组大肠杆菌胁迫抗性的影响。试验结果表明,经过热、酸胁迫后,重组大肠杆菌的存活率明显高于对照菌株,而在碱胁迫条件下,两种菌株的存活率相差不大,说明酸土脂环酸芽孢杆菌Dna J基因在大肠杆菌中异源表达能显著提高宿主菌对高温及酸胁迫的抗性,为发酵工业生产中构建高产抗逆工程菌株提供基因素材和理论依据。

It has constructed recombinant plasmids p ET28a-Dna J, transformed colon bacillus BL21 and has applied IPTG to induce recombinant Escherichia coli to express Dna J gene, and then researched the influence of Dna J gene expression on heat, acid and alkaline and stress resistance of recombinant Escherichia coli. The results indicated that survival rate of recombinant E. coli after heat and acid stress is obviously higher than control group, while survival rate of both groups is of little difference under alkaline stress, and indicates that heterologous expression of Dna J gene from Alicyclobacillus acidoterrestris in colon bacillus can significantly improve endurance for high temperature and acid stress of host strain. It has important application value to develop the new microorganisms with high production and preferable stress tolerant properties for main express in the production of fermentation industry.