优化提取鹅皮胶原蛋白肽及其层析分离产物的特性研究

Optimization of Extraction of Collagen Peptides from Goose Skin and Study on Chromatography Separations' Properties

探究了鹅皮胶原蛋白肽的提取条件,确定了其最佳提取工艺。采用水解度、DPPH自由基清除率和苦味值3个指标,得到最佳水解条件为木瓜蛋白酶:风味蛋白酶的比例1∶1,加酶量4 000 U/g,温度50℃,p H 6.0,水解时间5 h。优化后胶原蛋白肽的水解度达到28.3%,DPPH自由基清除率89.2%,苦味值0。通过Sephadex G-75层析分离得到4个组分,其中P4抗氧化性最强,对P4进行功能特性研究,结果表明其溶解性和乳化性都较好,可作为饮料中胶原多肽的良好来源。

The extraction condition of collagen peptides from goose skin was studied in this paper. The optimized procession was determined based on degree of hydrolysis（DH）, scavenging rate of DPPH· and bitterness. The optimum condition of extracting was as follows： the ratio of papain and flavourzyme was 1∶1, enzymes＇ dosage, temperature, p H,hydrolyzing time were 4 000 U·g-1, 50 ℃, 6.0 and 5 h respectively. DH of 28.3%, scavenging rate of DPPH· of 89.2%were achieved as a result with no bitterness. 4 fractions were separated and purified by Sephadex G-75 column chromatography and the strongest antioxidant activity was observed in P4. Solubility and emulsibility of P4 were tested finally and based on our experiments, we suggest that P4 could be added to drinks as an excellcent source of collagen peptides.