养心通脉有效部位方对大鼠骨髓间充质干细胞作用蛋白组学的生物信息学分析

Bioinformatics Analysis on Proteomics of Rat Bone Marrow Mesenchymal Stem Cells Intervened by Active Principle Region of Yang-Xin Tong-Mai Formula

目的:对养心通脉有效部位方(apr-YTF)应用于骨髓间充质干细胞(BMSCs)的作用蛋白组学蛋白进行生物信息学分析。方法:根据核素标记相对和绝对定量(i TRAQ)蛋白组学技术确定的apr-YTF对BMSCs作用靶点的差异蛋白,通过DAVID数据库搜索软件结合GO数据库搜索,进行注释和富集度分析,KEGG数据库进行信号转导通路分析;VISANT数据库检索蛋白质相互作用。结果:差异蛋白主要参与102种生物学过程、35种细胞组分、6种分子途径,差异蛋白主要参与3条信号转导通路,这些蛋白在3条信号转导通路上相互发生作用。结论:处于差异蛋白功能交互网中交叉点位置的Presenilin-1、Presenilin-2通过Notch信号通路,Syntaxin-4蛋白通过可溶性N-乙基顺丁烯二酰亚胺敏感性的融合蛋白附着蛋白(SNARE)信号通路,丝裂原活化蛋白激酶12(MAPK12)通过MAPK信号通路在apr-YTF诱导BMSCs向心肌分化过程中发挥重要作用,养心通脉有效部位方对骨髓间充质干细胞的作用是在多位点、多环节、多种生物学进程中发挥作用的,蛋白质组学生物信息学技术可以站在"整体观"的角度对中医药的作用机制作出预测,结合分子生物学验证是一条很好的中医药现代化途径。

This study was aimed to analyze the bioinformatics of proteomics of rat bone marrow mesenchymal stem cells （MSCs） intervened by active principle region of Yang-Xin Tong-Mai Formula （apr-YTF）. The latest versions of bioinformatics tools including DAVID （http：//david.abcc.ncifcrf.gov/） and GO （http：//www.geneontology.org/） were combined to assign a precise function to rat bone marrow MSCs intervened by apr-YTF. KEGG and VISANT were assigned with a precise function to rat bone marrow MSCs intervened by apr-YTF. The results showed that a total of 102 biological processes were mainly involved, with 35 cellular components and 6 molecular functions. These proteins interacted in 3 signal transduction pathways. It was concluded that the following proteins and signal transduction pathways played an important role in the process of apr-YTF inducing BMSCs differentiation into cardiomyocytes. Presenilin-1 and Presenilin-2 were in the Notch signaling pathway. And syntaxin-4 protein was in soluble N-ethylmaleimide sensitive fusion protein attachment protein （SNARE）. The apr-YTF played a role on MSCs from multiple sites, with multiple links through different biological processes. The bioinformatics of proteomics can predict action mechanism of traditional Chinese medicine （TCM） from the holism concept. The validation in combination with molecularbiology was a good way for TCM modernization.