摘要
目的:构建高表达抑癌基因Maspin的人胃癌MGC803细胞,研究不同浓度的南蛇藤提取物能否增强Maspin抑制胃癌细胞的侵袭能力。方法:克隆重组GV208-Maspin-EGFP慢病毒表达载体,感染人胃癌MGC803细胞。加入不同浓度的南蛇藤提取物(10、20、40μg·m L-1),Transwell小室法检测药物对高表达Maspin的人胃癌MGC803细胞侵袭能力的影响。结果:成功构建高表达Maspin的人胃癌MGC803细胞模型。各药物组Transwell小室下方穿膜细胞的数目减少,呈现一定的药物浓度依赖性。结论:南蛇藤提取物可增强抑癌基因Maspin抑制人胃癌细胞株MGC803侵袭的能力。
This study was aimed to investigate whether the extracts of Celastrus orbiculatus enhanced the invasion function of maspin tumor inhibitor gene through the construction of maspin overexpression human gastric carcinoma MGCS03 cell line. Maspin was cloned into plasmid GV208-EGFP eukaryotic expression vector. And then, the recombinant plasmid GV208-maspin-EGFP was transfected into human gastric carcinoma MGC803 cells. After the maspin overexpression MGC803 cell were treated with Celastrus orbiculatus extracts in different concentrations (10, 20, 40 μg·mL-1), the invasion effects were detected by Transwell chamber assay. The results showed that after the successful construction of maspin overexpression cell line, the number of cells invading through Matrigel was obviously decreased in the Transwell chamber assay. It also showed drug concentration dependency. It was concluded that maspin gene can inhibit invasion of gastric carcinoma MGCS03 cells. Simultaneously, the extracts of Celastrus orbiculatus can enhance the function of maspin gene.
出处
《世界科学技术-中医药现代化》
北大核心
2014年第11期2470-2474,共5页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金委青年项目(81403232):南蛇藤提取物靶向PI3K/Akt/m TOR信号通路抑制肝癌早期转移的作用及机制研究
负责人:钱亚云
国家自然科学基金委面上项目(81274141):南蛇藤总萜通过协同抑制Notch和VEGF的表达阻断肝癌血管生成的作用机制
负责人:刘延庆
江苏省自然科学基金委面上项目(BK2012686):南蛇藤提取物增强抑癌基因MASPIN表达抑制胃癌转移的机制研究
负责人:钱亚云
教育部博士点基金新教师类项目(20133250120003):南蛇藤总萜靶向m TOR信号通路抑制肝癌转移的分子机制研究
负责人:钱亚云
教育部大学生科创项目(201311117042):南蛇藤总萜靶向m TOR信号通路抑制肝癌转移的分子机制研究
负责人:吉兰芳
姜馨
江苏省教育厅大学生科创重点项目(201311117042z):南蛇藤总萜靶向m TOR信号通路抑制肝癌转移的分子机制研究
负责人:吉兰芳
姜馨
关键词
南蛇藤
MASPIN
胃癌
侵袭
Celastrus orbiculatus, maspin, gastric carcinoma, invasion