环孢菌素A体内作用2h对淋巴细胞亚群的影响

Two Hours Effects of Cyclosporin A on Lymphocyte Subsets in the Body

[目的]通过对急性髓细胞白血病移植患者服用环孢菌素A(cyclosporin A,Cs A)前后外周血淋巴细胞亚群的检测,初步探讨Cs A体内作用2h对淋巴细胞亚群的影响。[方法]采用流式细胞术检测43例急性髓细胞白血病移植后患者的空腹及服用Cs A 2h的外周血T、B淋巴细胞,NK细胞的表达;同时应用化学发光法检测Cs A的浓度。[结果]43例急性髓细胞白血病移植患者空腹Cs A浓度为(125.43±65.94)ng·m L-1,服药2h浓度为(564.46±258.67)ng·m L-1;服药2h与空腹相比,CD3+细胞显著下调而NK细胞显著上升,但CD4+、CD8+、CD19+细胞及CD4+/CD8+却无明显变化。[结论]服用Cs A 2h即能达到体内分布且影响CD3+细胞的数量,尚不能影响B细胞的数量。对于急性髓细胞白血病患者移植后服用Cs A期间,有必要对Cs A的浓度及淋巴细胞亚群进行监测以求达到合适的血药浓度,了解患者机体免疫状况,判断预后。

Objective] By detecting the peripheral blood lymphocyte subsets of AML patients before and after taking cyclosporin A, preliminary discussion on making two hours effects of cyclosporin A on lymphocyte subsets in the body. [Methods] Fasting and taking cyclosporin A two hours of the expression of peripheral blood T, B lymphocytes and NK cel were detected by flow cytometry in 43 cases of patients with AML after transplantation. At the same time, application of chemiluminescence detection concentration of cyclosporin A. [Results] The results of cyclosporin A concentration： Fasting group is 125.43 ±65.94）ng·ml-1. Taking two hours group is 564.46±258.67 ng·ml-1; Taking two hours group compared with the fasting group, CD3＋ cel is significantly reduced and NK cel is significantly increased, but CD4＋, CD8＋, CD19＋cel and CD4＋/CD8＋ have no obvious change. [Conclusions] Taking cyclosporin A 2 hours can achieve distribution and affect the number of CD3＋ cel in the body, but can not affect the B cel . To AML patients after transplantation, in order to achieve the appropriate concentration and keep abreast of the body＇s immune status of patients, it is necessary to monitor the concentration of cyclosporin A and lymphocyte subsets during their taking cyclosporin A, which helps us determine the prognosis.