Th1型和Th2型细胞因子在口腔扁平苔藓患者外周血中的表达及临床意义

Effect and Clinical Significance of Expression of Th1 and Th2 in Oral Lichen Planus

目的:研究口腔扁平苔藓(OLP1)患者Th1型和Th2型细胞因子的表达及临床意义。方法:选取2013年1月至2014年3月于我院就诊的21例充血糜烂型及14例光滑型OLP患者为研究对象,18例正常人为对照组,采用密度梯度离心法对各组外周血单个核细胞进行分离,酶联免疫吸附剂测定(ELISA)法对各组外周血单个核细胞中的IL-4和IFN-gamma的表达进行检测,逆转录-聚合酶链反应法对各组血清中IL-4 m RNA和IFN-gamma m RNA的表达进行检测。结果:与正常对照组相比,OLP患者IL-4m RNA及蛋白的表达均增高,而IFN-gamma m RNA及蛋白的表达则降低,差异均有显著统计学意义(均P<0.01)。充血糜烂型及光滑型OLP患者组间比较发现,IL-4 m RNA和IFN-gamma m RNA及蛋白的表达差异无统计学意义(P>0.05)。结论:OLP发病机制与Th1与Th2的表达失衡有关,为临床治疗提供参考。

Objective： To evaluate the expression of Th1 and Th2 in peripheral blood of oral lichen planus（OLP） and its clinical significance. Methods： 21 cases of hyperaemia erosion OLP and 14 cases of smooth OLP patients in our hospital from January 2013 to March 2014 were researched and 18 healthy people were studied as control group. The peripheral blood mononuclear cells were isolated by density gradient centrifugation, the expression of IL-4 m RNA and IFN-gamma m RNA in peripheral blood mononuclear cells was detected by reverse transcription polymerase chain reaction method and IL-4 and IFN-gamma were tested by ELISA. Results： The expression of IL-4 m RNA and the protein was higher in OLP group than in control group, expression of IL-4 m RNA and the protein was lower in OLP group with a statistically significant difference（all P〈0.01）. However, there were no significant differences in the expression of IL-4 m RNA, IFN-gamma m RNA and protein between hyperaemia erosion and smooth OLP patients（P〉0.05）. Conclusion：The pathogenesis of OLP involves the unbalanced expression of Th1 and Th2, and it provides the reference for the clinical treatment.