骨髓基质细胞IL-1β、IL-6、SCF及TPO在老年多发性骨髓瘤组织中的表达及意义

Clinical Significance and Determination of IL-1β, IL-6, SCF and TPO of Marrow Stromal Cells in Elderly Patients with Multiple Myeloma

目的:探讨老年多发性骨髓瘤患者骨髓基质细胞白细胞介素-1(IL-1β)、白细胞介素-6(IL-6)、肝细胞因子(SCF)、血小板生成素(TPO)水平及临床意义。方法:选择本院2011年1月-2014年6月收治的老年多发性骨髓瘤患者作为观察组,另选择同期参加体检的志愿者作为对照组。采集两组人员骨髓标本,制备总RNA、反转录反应、聚合酶链反应及PCR产物电泳检测等过程的测定两组患者IL-1β、IL-6、SCF、TPO水平。结果:观察组患者IL-1β、IL-6、SCF、TPO水平均明显高于对照组(P<0.05);初发患者、复发患者及移植患者的IL-1β、IL-6比较无明显差异(P>0.05)。移植组SCF水平显著低于初发组和复发组(t初移=4.967,t难移=5.169,P<0.05);初发组TPO显著高于复发组和移植组(t初难=4.736,t初移=3.568,P<0.05)。结论:老年多发性骨髓瘤患者骨髓基质细胞白细胞介素-1、白细胞介素-6、肝细胞因子、血小板生成素水平表达升高,在疾病的发生及发展过程中发挥重要作用。

Objective： To investigate the levels of interleukin-1（IL-1 β）, interleukin-6（IL-6）, stem cell factor（SCF）, and thrombopoietin（TPO） in elderly multiple myeloma patients stromal cells. Methods： 13 cases of elderly multiple myeloma patients treated in the hospital from January 2011 to June 2014 were selected as the observation group, while another 13 healthy people were collected as the control group. Then the levels of IL-1 β, IL-6, SCF and TPO of the two groups were detected by means of RNA, reverse transcription,polymerase chain reaction and PCR. Results： The IL-1β, IL-6, SCF and TPO in the observation group were significantly higher than those of the control group（P〈0.05）; There was no significant difference about IL-1β and IL-6 in the three groups（P〉0.05）; SCF levels in the transplantation group was significantly lower than those of the primary group and refractory group（t=4.967, t=5.169, P〈0.05）; TPO levels of the primary group was significantly higher than those of the refractory group and transplantation group（t=4.736, t=3.568, P〈0.05）. Conclusion： The interleukin-1, interleukin-6, stem cell factor and thrombopoietin may play an important role in the development of multiple myeloma for elderly patients.