胆石患者术后胆盐转运子BSEP、MRP2、NTCP的水平变化及意义

Expression and Significance of Bile Salt Transporter Factors BSEP, MRP2,NTCP in Patients with Gallbladder Stone after Laparoscopic Joint Choledochoscope Surgery

目的:探讨腹腔镜联合胆道镜微创手术对胆囊结石患者胆盐转运因子胆盐输出泵(BSEP)、多重耐药蛋白2(MRP2)和牛黄胆酸钠转运蛋白(NTCP)水平的影响。方法:选取我院普外科收治的胆囊结石患者20例排除手术和麻醉禁忌症,予腹腔镜联合胆道镜微创手术治疗。治疗结束后,对比治疗前后患者BSEP、MRP2、NTCP水平变化。结果:1治疗后患者肝脏组织中BSEP、MRP2水平明显比治疗前升高,差异有统计学意义(P<0.05);2治疗后患者肝组织中NTCP水平与治疗前水平无明显变化,差异无统计学意义(P>0.05)。结论:腹腔镜联合胆道镜微创手术治疗胆囊结石能升高患者胆盐转运因子BSEP、MRP2水平,提高患者术后对胆盐的转运和胆汁酸的代谢,降低胆囊结石复发率,对临床具有指导意义,值得临床推广。

Objective： To investigate the change of bile salt transporter factors bile salt export pump（BSEP）, multiple drug-resistant proteins 2（MRP2） and cow bile acid sodium transporters（NTCP） levels in patients with gallbladder stone after laparoscopic joint choledochoscope minimally invasive surgery. Methods： 20 patients with gallbladder stones without surgery and anesthesia contraindications were selected from department of general surgery. Then the joint choledochoscope to laparoscopic minimally invasive surgery was performed. The change of BSEP, MRP2, NTCP levels in liver tissues of patients was detected and compared before and after treatment. Results：（1） After treatment, the transcript and protein level of BSEP, MRP2 was significantly higher than before treatment, the difference was statistically significant（P〈 0.05）;（2） Before and after treatment, no obvious change in NTCP m RNA level was detected（P〉 0.05）. Conclusion： Laparoscopic joint choledochoscope minimally invasive surgery can increase bile salt transfer factors BSEP, MRP2 level in patients with gallbladder stone, improve postoperative transport of bile salt and metabolism of bile acid, and reduce the recurrence rate of gall bladder calculi, which is worthy of clinical promotion.