长波紫外光引起人红细胞自体荧光增强效应的研究

Study of Autofluorescence Enhancement Effects in Human Erythrocytes Induced by Long-wave Ultraviolet Irradiation

研究长波紫外光对红细胞的生物学效应之一——自荧光增强效应。用不同强度长波紫外光(365 nm)照射人红细胞,观察红细胞自体荧光变化。在实验中加入外源活性氧理论模拟非连续紫外光引起自荧光变化。结果随着照射强度的升高,红细胞自荧光作用逐渐加强;随着时间延长荧光逐渐减弱。连续紫外光照射下,起始自荧光增强速率是受到细胞内氧自由基浓度的影响;因此,在过程中紫外光的作用之一是生成大量活性氧,进一步影响自荧光增强的速率。在紫外线照射充氧自血回输疗法(UBIO疗法)过程中,对血液进行短时间的紫外光照射,必然会使红细胞中的血红素发生光解,并生成了一定量的活性氧自由基(ROS),再将红细胞回输人体后可能引起人体内的应激反应,从而激发清除这些产物的一系列反应,从而改善人体环境。

To investigate long-wave ultraviolet irradiation（ UVI）-induced autofluorescence enhancement effects in human erythrocytes,erythrocytes were irradiated by UV light（ 365 nm） with different irradiation intensity and manner in order to observe the photodynamic process of autofluorescence enhancement. We sought to experimentally and theoretically analyze the effects of reactive oxygen species（ ROS） on this autofluorescence enhancement. The data showed that UVI resulted in autofluorescence enhancement of erythrocytes in a intensity-dependent manner.The time of arrival in fluorescence peak was associated with the irradiation dose. Besides,the experimental and theoretical results indicated that ROS played a key role in the autofluorescence enhancement process in a concentration-dependent manner. During the ultraviolet blood irradiation and oxygenation（ UBIO） therapy,UVI caused a certain production of ROS due to the photodynamic effects which in turn activated a series of stress response,resulting in improvement of human health.