摘要
研究产电微生物胞外电子传递过程和机制,发现与产电效率相关的关键基因、通路和代谢物,是微生物燃料电池研究中的关键技术。为了发现在胞外电子传递过程中起到关键作用的基因以及通路,首先利用比较基因组学的方法,以模式微生物大肠杆菌和同属希瓦氏菌的其他菌株为参考,构建了Shewanella.onedensis MR-1的全基因组基因转录调控网络,大大扩展了目前已知的基因调控关系。然后以此网络为基础,结合基于蛋白质相互作用分析得到的胞外电子传递通路,构建了与胞外电子传递直接传递密切相关的细胞色素C编码基因及其相关调控基因构成的子网络,结合全基因组基因表达数据,研究了特异性条件下胞外电子传递的可能通路和基因调控过程。
To improve the efficiency of electricity production of MFC, it is necessary to understand the principles of extracellular electron transfer(EET) of Shewanella oneidensis MR-1, for example, the EET pathways under specific conditions( different grow conditions or different terminal electron acceptor and so on). 41 c-type cytochromes of Shewanella oneidensis MR-1 were obtained from literatures and 50 related proteins were downloaded in STRING, then the coding genes of these proteins in NCBI. As the GRN of Shewanella oneidensis MR-1 had been reconstructed before, and 3367 transcription relationships were discovered, in which the transcription relationships between c-type cytochromes related genes were identified. Using R platform, we built the gene regulatory networks and explored the possible pathways to clarify the principles of EET, the bacterial growth conditions were controlled by using different gene expression datasets. At last, three specific EET oathwavs were orooosed while analvzing GSE7973. GSE15334 and GSE25821.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2014年第11期42-46,共5页
China Biotechnology