聚合抗稻瘟病基因Pi9和抗褐飞虱基因Bph18(t)选育水稻恢复系

Pyramiding the Blast Resistant Gene Pi9 and the Brown Planthopper Gene Bph18(t) to Develop Restorer Lines in Rice(Oryza sativa L.)

水稻(Oryza sativa L.)作为粮食作物,在全世界粮食生产中具有极其重要的地位。稻瘟病、褐飞虱等病虫害的发生严重危害着水稻的安全生产,培育并利用水稻抗性品种能经济有效地预防水稻病虫害的发生,因此,培育抗性品种在水稻安全生产中尤为重要。本研究以具有抗稻瘟病基因Pi9的抗病品系75-1-127为抗病亲本,以携带有抗褐飞虱基因Bph18(t)的水稻材料C4064为抗虫亲本,以性状优良的恢复系测679作为轮回亲本,进行杂交、回交和自交,并辅以田间多代选择。在分离群体中,使用与Pi9紧密连锁的SCAR标记p B8跟踪目标基因Pi9,利用与Bph18(t)紧密连锁的标记KC16跟踪目标基因Bph18(t)。通过分子标记辅助选择、农艺性状评价和抗病抗虫鉴定,选育出聚合了Pi9和Bph18(t)基因、对稻瘟病和褐飞虱的抗性水平接近抗病亲本或抗虫亲本的恢复系。

Rice （Oryza sativa L.）, a cereal crop, plays an ultimately important role in the global food production. The occurrence of the rice disease and pest such as the rice blast （RB） and the brown planthopper （BPH） can seve- rely damage the safe production of rice, and the development and application of the resistant varieties can prevent the occurrence of the rice disease and pest economically and effectively, so the development of the resistant variety is crucial in the safe production of rice. In this study, we used the disease resistant line 75-1-127 carrying rice blast resistant gene Pi9 as the disease-resistant parent, the BPH-resistant line C4064 harboring brown planthopper resistant gene Bph18（t） as the BPH-resistant parent, and the elite restoring line Ce679 as recurrent parents, to hy- bridize, backcross, selfcross and field select for multiple generations. The Pi9-1inked SCAR marker pB8 was used to select Pi9, and the Bph18（t）-linked marker KC16 was used to select Bph18（t） in the segregating populations. By molecular marker-assisted selection, evaluation of agronomic traits and assay of resistance, we developed restorer lines which pyramided Pi9 and Bph18（t） genes, and which demonstrated the similar level of resistance against rice blast and brown planthopper as compared with the disease-resistant parent or the BPH-resistant parent.